Light and electron microscopic features of peripheral ganglion cells cultured from young and aged Wistar rats

Jari Koistinaho, Kimmo Hatanpää, Antti Hervonen

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Neurons of the dorsal root ganglion (DRG) and sympathetic superior cervical ganglion (SCG) were cultured as explants from young adult (3 months old) and aged (28 months old) Wistar rats. Both aged DRG and SCG neurons showed delayed neurite outgrowth compared to young adult neurons. Young and some aged cultured neruons had an ultrastructure similar to that found in normal uncultured cells, but most of the aged cultured neurons displayed a heavy accumulation of hemogenous lipid-like inclusions in addition to classic age pigments. Occasionally, large neurofilament aggregates were seen in aged DRG neurons. They were sometimes localized perinuclearly, resembling neurofibrillary tangles. The results show that even very old peripheral neurons survive in culture. As aged cultured neurons show degenerative changes not observed in young adult neurons, it is suggested that cultured peripheral neurons of different ages could provided useful means for neuronal aging studies.

Original languageEnglish (US)
Pages (from-to)23-32
Number of pages10
JournalMechanisms of Ageing and Development
Volume61
Issue number1
DOIs
StatePublished - Nov 15 1991

Keywords

  • Aging
  • In culture
  • Lipofuscin
  • Peripheraal neuron
  • Ultrastructure

ASJC Scopus subject areas

  • Aging
  • Developmental Biology

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