Light-independent phosphorylation of WHITE COLLAR-I regulates its function in the Neurospora circadian negative feedback loop

Qiyang He, Hongjun Shu, Ping Cheng, She Chen, Lixin Wang, Yi Liu

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Phosphorylation is a major regulatory mechanism controlling circadian clocks. In the Neurospora circadian clock, the PER-AKNT-SIM (PAS) domain-containing transcription factor, WHITE COLLAR (WC)-1, acts both as the blue light photoreceptor of the clock and as a positive element in the circadian negative feedback loop in constant darkness, by activating the transcription of the frequency (frq) gene. To understand the role of WC-1 phosphorylation, five in vivo WC-1 phosphorylation sites, located immediately downstream of the WC-1 zinc finger DNA binding domain, were identified by tandem mass spectrometry using biochemically purified endogenous WC-1 protein. Mutations of these phosphorylation sites suggest that they are major WC-1 phosphorylation sites under constant conditions but are not responsible for the light-induced hyperphosphorylation of WC-1. Although phosphorylation of these sites does not affect the light function of WC-1, strains carrying mutations of these sites show short period, low amplitude, or arrhythmic conidiation rhythms in constant darkness. Furthermore, normal or slightly higher levels of frq mRNA and FRQ proteins were observed in a mutant strain containing mutations of all five sites despite its low WC-1 levels. Together, these data suggest that phosphorylation of these sites negatively regulates the function of WC-1 in the circadian negative feedback loop and is important for the function of the Neurospora circadian clock.

Original languageEnglish (US)
Pages (from-to)17526-17532
Number of pages7
JournalJournal of Biological Chemistry
Volume280
Issue number17
DOIs
StatePublished - Apr 29 2005

Fingerprint

Neurospora
Phosphorylation
Feedback
Light
Circadian Clocks
Clocks
Darkness
Mutation
Zinc Fingers
Transcription
Tandem Mass Spectrometry
Gene Frequency
Mass spectrometry
Zinc
Proteins
Transcription Factors
Genes
Messenger RNA
DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Light-independent phosphorylation of WHITE COLLAR-I regulates its function in the Neurospora circadian negative feedback loop. / He, Qiyang; Shu, Hongjun; Cheng, Ping; Chen, She; Wang, Lixin; Liu, Yi.

In: Journal of Biological Chemistry, Vol. 280, No. 17, 29.04.2005, p. 17526-17532.

Research output: Contribution to journalArticle

He, Qiyang ; Shu, Hongjun ; Cheng, Ping ; Chen, She ; Wang, Lixin ; Liu, Yi. / Light-independent phosphorylation of WHITE COLLAR-I regulates its function in the Neurospora circadian negative feedback loop. In: Journal of Biological Chemistry. 2005 ; Vol. 280, No. 17. pp. 17526-17532.
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