Linkage analysis of variations in CD4: CD8 T cell subsets between C57BL/6 and DBA/2

C. Myrick, R. DiGuisto, J. DeWolfe, E. Bowen, J. Kappler, P. Marrack, Edward K. Wakeland

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The ratio of CD4 T cells to CD8 T cells (CD4:CD8 ratio) varies over two-fold between C57BL/6 and DBA/2 mice for both T cell precursors in the thymus and mature T cells in the periphery. Correlation analysis of the CD4:CD8 ratio in thymic precursors vs peripheral T cells in F2 and backcross mice found that thymic precursor ratios are inherited independently from those in the periphery, indicating that the CD4:CD8 ratios in these populations are affected by distinct genetic mechanisms. A genome scan of progeny in the phenotypic extremes identified three quantitative trait loci (QTLs). Trmq1 (for T cell ratio modifier QTL 1) was detected in the telomeric end of c6 (peak marker D6Mit15 at 74 cM) and had a maximum LOD score of 4.6. Trmq2, in the telomeric half of c2, peaked at D2MIT483 and had a maximum LOD score of 3.41. Both of these QTLs impacted the CD4:CD8 ratios in peripheral T cells and had no impact on variation in this ratio among thymic precursors. However, heterozygosity for the H2 complex was suggestively associated (LOD score of 2.43) with increases in CD4 T cells among T cell precursors in the thymus. All of these QTLs were affected by epistatic interactions, indicating that additional modifiers in the B6 and DBA/2 genomes modulate this phenotype.

Original languageEnglish (US)
Pages (from-to)144-150
Number of pages7
JournalGenes and Immunity
Volume3
Issue number3
DOIs
StatePublished - May 2002

Fingerprint

T-Lymphocyte Subsets
CD4-CD8 Ratio
T-Lymphocytes
Quantitative Trait Loci
T-Lymphoid Precursor Cells
Thymus Gland
Genome
Inbred DBA Mouse
Phenotype
Population

Keywords

  • CD4 T cell subsets
  • CD8 T cell subsets
  • Epistasis
  • Genome scan
  • Quantitative trait loci
  • T cells

ASJC Scopus subject areas

  • Genetics(clinical)
  • Immunology
  • Genetics

Cite this

Linkage analysis of variations in CD4 : CD8 T cell subsets between C57BL/6 and DBA/2. / Myrick, C.; DiGuisto, R.; DeWolfe, J.; Bowen, E.; Kappler, J.; Marrack, P.; Wakeland, Edward K.

In: Genes and Immunity, Vol. 3, No. 3, 05.2002, p. 144-150.

Research output: Contribution to journalArticle

Myrick, C, DiGuisto, R, DeWolfe, J, Bowen, E, Kappler, J, Marrack, P & Wakeland, EK 2002, 'Linkage analysis of variations in CD4: CD8 T cell subsets between C57BL/6 and DBA/2', Genes and Immunity, vol. 3, no. 3, pp. 144-150. https://doi.org/10.1038/sj.gene.6363819
Myrick C, DiGuisto R, DeWolfe J, Bowen E, Kappler J, Marrack P et al. Linkage analysis of variations in CD4: CD8 T cell subsets between C57BL/6 and DBA/2. Genes and Immunity. 2002 May;3(3):144-150. https://doi.org/10.1038/sj.gene.6363819
Myrick, C. ; DiGuisto, R. ; DeWolfe, J. ; Bowen, E. ; Kappler, J. ; Marrack, P. ; Wakeland, Edward K. / Linkage analysis of variations in CD4 : CD8 T cell subsets between C57BL/6 and DBA/2. In: Genes and Immunity. 2002 ; Vol. 3, No. 3. pp. 144-150.
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