Lipid metabolism and adipokine levels in fatty acid-binding protein null and transgenic mice

Ann V. Hertzel, Lisa Ann Smith, Anders H. Berg, Gary W. Cline, Gerald I. Shulman, Philipp E. Scherer, David A. Bernlohr

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Fatty acid-binding proteins (FABPs) facilitate the diffusion of fatty acids within cellular cytoplasm. Compared with C57Bl/6J mice maintained on a high-fat diet, adipose-FABP (A-FABP) null mice exhibit increased fat mass, decreased lipolysis, increased muscle glucose oxidation, and attenuated insulin resistance, whereas overexpression of epithelial-FABP (EFABP) in adipose tissue results in decreased fat mass, increased lipolysis, and potentiated insulin resistance. To identify the mechanisms that underlie these processes, real-time PCR analyses indicate that the expression of hormone-sensitive lipase is reduced, while perilipin A is increased in A-FABP/aP2 null mice relative to E-FABP overexpressing mice. In contrast, de novo lipogenesis and expression of genes encoding lipoprotein lipase, CD36, long-chain acyl-CoA synthetase 5, and diacylglycerol acyltransferase are increased in A-FABP/aP2 null mice relative to EFABP transgenic animals. Consistent with an increase in de novo lipogenesis, there was an increase in adipose C16:0 and C16:1 acyl-CoA pools. There were no changes in serum free fatty acids between genotypes. Serum levels of resistin were decreased in the E-FABP transgenic mice, whereas serum and tissue adiponectin were increased in A-FABP/aP2 null mice and decreased in E-FABP transgenic animals; leptin expression was unaffected. These results suggest that the balance between lipolysis and lipogenesis in adipocytes is remodeled in the FABP null and transgenic mice and is accompanied by the reprogramming of adipokine expression in fat cells and overall changes in plasma adipokines.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume290
Issue number5
DOIs
StatePublished - May 2006

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Fatty Acid-Binding Proteins
Adipokines
Lipid Metabolism
Transgenic Mice
Lipogenesis
Lipolysis
Fats
Acyl Coenzyme A
Genetically Modified Animals
Adipocytes
Insulin Resistance
Animals
Serum
Diacylglycerol O-Acyltransferase
Insulin
Tissue
Sterol Esterase
Resistin
Gene encoding
Lipoprotein Lipase

Keywords

  • Adipocytes
  • Adipokines
  • Fatty acids

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry

Cite this

Lipid metabolism and adipokine levels in fatty acid-binding protein null and transgenic mice. / Hertzel, Ann V.; Smith, Lisa Ann; Berg, Anders H.; Cline, Gary W.; Shulman, Gerald I.; Scherer, Philipp E.; Bernlohr, David A.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 290, No. 5, 05.2006.

Research output: Contribution to journalArticle

Hertzel, Ann V. ; Smith, Lisa Ann ; Berg, Anders H. ; Cline, Gary W. ; Shulman, Gerald I. ; Scherer, Philipp E. ; Bernlohr, David A. / Lipid metabolism and adipokine levels in fatty acid-binding protein null and transgenic mice. In: American Journal of Physiology - Endocrinology and Metabolism. 2006 ; Vol. 290, No. 5.
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