Lipid modifications of G proteins

α subunits are palmitoylated

Maurine E. Linder, Pamela Middleton, John R. Hepler, Ronald Taussig, Alfred G. Gilman, Susanne M. Mumby

Research output: Contribution to journalArticle

275 Citations (Scopus)

Abstract

A small number of membrane-associated proteins are reversibly and covalently modified with palmitic acid. Palmitoylation of G-protein α and β subunits was assessed by metabolic labeling of subunits expressed in simian COS cells or insect Sf9 cells. The fatty acid was incorporated into all of the α subunits examined (αs, αo, αi1, αi2, αi3, αz, and αq), including those that are also myristoylated (αo, αi, and αz). Palmitate was released by treatment with base, suggesting attachment to the protein through a thioester or ester bond. Limited tryptic digestion of activated αo and αs resulted in release of the ammo-terminal portions of the proteins and radioactive palmitate. These data are consistent with palmitoylation of the proteins near their amino termini, most likely on Cys-3. Reversible acylation of G-protein α subunits may provide an additional mechanism for regulation of signal transduction.

Original languageEnglish (US)
Pages (from-to)3675-3679
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number8
StatePublished - Apr 15 1993

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Protein Subunits
GTP-Binding Proteins
Lipoylation
Palmitates
Lipids
Sf9 Cells
Proteins
Acylation
Palmitic Acid
COS Cells
Insects
Digestion
Signal Transduction
Membrane Proteins
Esters
Fatty Acids

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Linder, M. E., Middleton, P., Hepler, J. R., Taussig, R., Gilman, A. G., & Mumby, S. M. (1993). Lipid modifications of G proteins: α subunits are palmitoylated. Proceedings of the National Academy of Sciences of the United States of America, 90(8), 3675-3679.

Lipid modifications of G proteins : α subunits are palmitoylated. / Linder, Maurine E.; Middleton, Pamela; Hepler, John R.; Taussig, Ronald; Gilman, Alfred G.; Mumby, Susanne M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 8, 15.04.1993, p. 3675-3679.

Research output: Contribution to journalArticle

Linder, Maurine E. ; Middleton, Pamela ; Hepler, John R. ; Taussig, Ronald ; Gilman, Alfred G. ; Mumby, Susanne M. / Lipid modifications of G proteins : α subunits are palmitoylated. In: Proceedings of the National Academy of Sciences of the United States of America. 1993 ; Vol. 90, No. 8. pp. 3675-3679.
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