TY - JOUR
T1 - Lipid-protein interactions drive membrane protein topogenesis in accordance with the positive inside rule
AU - Bogdanov, Mikhail
AU - Xie, Jun
AU - Dowhan, William
PY - 2009/4/10
Y1 - 2009/4/10
N2 - Transmembrane domain orientation within some membrane proteins is dependent on membrane lipid composition. Initial orientation occurs within the translocon, but final orientation is determined after membrane insertion by interactions within the protein and between lipid headgroups and protein extramembrane domains. Positively and negatively charged amino acids in extramembrane domains represent cytoplasmic retention and membrane translocation forces, respectively, which are determinants of protein orientation. Lipids with no net charge dampen the translocation potential of negative residues working in opposition to cytoplasmic retention of positive residues, thus allowing the functional presence of negative residues in cytoplasmic domains without affecting protein topology.
AB - Transmembrane domain orientation within some membrane proteins is dependent on membrane lipid composition. Initial orientation occurs within the translocon, but final orientation is determined after membrane insertion by interactions within the protein and between lipid headgroups and protein extramembrane domains. Positively and negatively charged amino acids in extramembrane domains represent cytoplasmic retention and membrane translocation forces, respectively, which are determinants of protein orientation. Lipids with no net charge dampen the translocation potential of negative residues working in opposition to cytoplasmic retention of positive residues, thus allowing the functional presence of negative residues in cytoplasmic domains without affecting protein topology.
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U2 - 10.1074/jbc.R800081200
DO - 10.1074/jbc.R800081200
M3 - Short survey
C2 - 19074771
AN - SCOPUS:65649102996
SN - 0021-9258
VL - 284
SP - 9637
EP - 9641
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 15
ER -