Lipid-protein interactions drive membrane protein topogenesis in accordance with the positive inside rule

Mikhail Bogdanov, Jun Xie, William Dowhan

Research output: Contribution to journalShort surveypeer-review

54 Scopus citations

Abstract

Transmembrane domain orientation within some membrane proteins is dependent on membrane lipid composition. Initial orientation occurs within the translocon, but final orientation is determined after membrane insertion by interactions within the protein and between lipid headgroups and protein extramembrane domains. Positively and negatively charged amino acids in extramembrane domains represent cytoplasmic retention and membrane translocation forces, respectively, which are determinants of protein orientation. Lipids with no net charge dampen the translocation potential of negative residues working in opposition to cytoplasmic retention of positive residues, thus allowing the functional presence of negative residues in cytoplasmic domains without affecting protein topology.

Original languageEnglish (US)
Pages (from-to)9637-9641
Number of pages5
JournalJournal of Biological Chemistry
Volume284
Issue number15
DOIs
StatePublished - Apr 10 2009

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Lipid-protein interactions drive membrane protein topogenesis in accordance with the positive inside rule'. Together they form a unique fingerprint.

Cite this