Lipid utilization in skeletal muscle cells is modulated in vitro and in vivo by specific miRNAs

Francesco Chemello; Francesca Grespi; Alessandra Zulian; Pasqua Cancellara; Etienne Hebert-Chatelain; Paolo Martini; Camilla Bean; Enrico Alessio; Ruggero Ferrazza; Paolo Laveder; Graziano Guella; Carlo Reggiani; Chiara Romualdi; Paolo Bernardi; Luca Scorrano; Stefano Cagnin; Gerolamo Lanfranchi

doi:10.1101/288035

Lipid utilization in skeletal muscle cells is modulated in vitro and in vivo by specific miRNAs

Francesco Chemello, Francesca Grespi, Alessandra Zulian, Pasqua Cancellara, Etienne Hebert-Chatelain, Paolo Martini, Camilla Bean, Enrico Alessio, Ruggero Ferrazza, Paolo Laveder, Graziano Guella, Carlo Reggiani, Chiara Romualdi, Paolo Bernardi, Luca Scorrano, Stefano Cagnin, Gerolamo Lanfranchi

Research output: Contribution to journal › Article › peer-review

Abstract

Skeletal muscle is composed by different myofiber types that can preferentially use glycolysis or lipids for ATP production. How fuel preference is specified in these post-mitotic cells is unknown. Here we show that miRNAs are important players in defining the myofiber metabolic profile. mRNA and miRNA signatures of all myofiber types obtained at single cell level unveiled fiber-specific regulatory networks and identified two master miRNAs that coordinately control myofiber fuel preference and mitochondrial morphology. Our work provides a complete and integrated myofiber type-specific catalogue of genes and miRNAs expressed and establishes miR-27a-3p and miR-142-3p as key regulators of lipid utilization in skeletal muscle.

Original language	English (US)
Journal	Unknown Journal
DOIs	https://doi.org/10.1101/288035
State	Published - Mar 23 2018
Externally published	Yes

Keywords

Lipids
MiRNAs
Mitochondria
Single myofiber
Skeletal muscle metabolism

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
Immunology and Microbiology(all)
Neuroscience(all)
Pharmacology, Toxicology and Pharmaceutics(all)

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10.1101/288035

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Chemello, F., Grespi, F., Zulian, A., Cancellara, P., Hebert-Chatelain, E., Martini, P., Bean, C., Alessio, E., Ferrazza, R., Laveder, P., Guella, G., Reggiani, C., Romualdi, C., Bernardi, P., Scorrano, L., Cagnin, S., & Lanfranchi, G. (2018). Lipid utilization in skeletal muscle cells is modulated in vitro and in vivo by specific miRNAs. Unknown Journal. https://doi.org/10.1101/288035

Lipid utilization in skeletal muscle cells is modulated in vitro and in vivo by specific miRNAs. / Chemello, Francesco; Grespi, Francesca; Zulian, Alessandra; Cancellara, Pasqua; Hebert-Chatelain, Etienne; Martini, Paolo; Bean, Camilla; Alessio, Enrico; Ferrazza, Ruggero; Laveder, Paolo; Guella, Graziano; Reggiani, Carlo; Romualdi, Chiara; Bernardi, Paolo; Scorrano, Luca; Cagnin, Stefano; Lanfranchi, Gerolamo.

In: Unknown Journal, 23.03.2018.

Research output: Contribution to journal › Article › peer-review

Chemello, F, Grespi, F, Zulian, A, Cancellara, P, Hebert-Chatelain, E, Martini, P, Bean, C, Alessio, E, Ferrazza, R, Laveder, P, Guella, G, Reggiani, C, Romualdi, C, Bernardi, P, Scorrano, L, Cagnin, S & Lanfranchi, G 2018, 'Lipid utilization in skeletal muscle cells is modulated in vitro and in vivo by specific miRNAs', Unknown Journal. https://doi.org/10.1101/288035

Chemello, Francesco ; Grespi, Francesca ; Zulian, Alessandra ; Cancellara, Pasqua ; Hebert-Chatelain, Etienne ; Martini, Paolo ; Bean, Camilla ; Alessio, Enrico ; Ferrazza, Ruggero ; Laveder, Paolo ; Guella, Graziano ; Reggiani, Carlo ; Romualdi, Chiara ; Bernardi, Paolo ; Scorrano, Luca ; Cagnin, Stefano ; Lanfranchi, Gerolamo. / Lipid utilization in skeletal muscle cells is modulated in vitro and in vivo by specific miRNAs. In: Unknown Journal. 2018.

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abstract = "Skeletal muscle is composed by different myofiber types that can preferentially use glycolysis or lipids for ATP production. How fuel preference is specified in these post-mitotic cells is unknown. Here we show that miRNAs are important players in defining the myofiber metabolic profile. mRNA and miRNA signatures of all myofiber types obtained at single cell level unveiled fiber-specific regulatory networks and identified two master miRNAs that coordinately control myofiber fuel preference and mitochondrial morphology. Our work provides a complete and integrated myofiber type-specific catalogue of genes and miRNAs expressed and establishes miR-27a-3p and miR-142-3p as key regulators of lipid utilization in skeletal muscle.",

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AU - Cancellara, Pasqua

AU - Hebert-Chatelain, Etienne

AU - Martini, Paolo

AU - Bean, Camilla

AU - Alessio, Enrico

AU - Ferrazza, Ruggero

AU - Laveder, Paolo

AU - Guella, Graziano

AU - Reggiani, Carlo

AU - Romualdi, Chiara

AU - Bernardi, Paolo

AU - Scorrano, Luca

AU - Cagnin, Stefano

AU - Lanfranchi, Gerolamo

N1 - Publisher Copyright: The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2018/3/23

Y1 - 2018/3/23

N2 - Skeletal muscle is composed by different myofiber types that can preferentially use glycolysis or lipids for ATP production. How fuel preference is specified in these post-mitotic cells is unknown. Here we show that miRNAs are important players in defining the myofiber metabolic profile. mRNA and miRNA signatures of all myofiber types obtained at single cell level unveiled fiber-specific regulatory networks and identified two master miRNAs that coordinately control myofiber fuel preference and mitochondrial morphology. Our work provides a complete and integrated myofiber type-specific catalogue of genes and miRNAs expressed and establishes miR-27a-3p and miR-142-3p as key regulators of lipid utilization in skeletal muscle.

AB - Skeletal muscle is composed by different myofiber types that can preferentially use glycolysis or lipids for ATP production. How fuel preference is specified in these post-mitotic cells is unknown. Here we show that miRNAs are important players in defining the myofiber metabolic profile. mRNA and miRNA signatures of all myofiber types obtained at single cell level unveiled fiber-specific regulatory networks and identified two master miRNAs that coordinately control myofiber fuel preference and mitochondrial morphology. Our work provides a complete and integrated myofiber type-specific catalogue of genes and miRNAs expressed and establishes miR-27a-3p and miR-142-3p as key regulators of lipid utilization in skeletal muscle.

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KW - Skeletal muscle metabolism

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