Lipidomic and Transcriptomic Basis of Lysosomal Dysfunction in Progranulin Deficiency

Bret M. Evers, Carlos Rodriguez-Navas, Rachel J. Tesla, Janine Prange-Kiel, Catherine R. Wasser, Kyoung Shin Yoo, Jeffrey McDonald, Basar Cenik, Thomas A. Ravenscroft, Florian Plattner, Rosa Rademakers, Gang Yu, Charles L. White, Joachim Herz

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Defective lysosomal function defines many neurodegenerative diseases, such as neuronal ceroid lipofuscinoses (NCL) and Niemann-Pick type C (NPC), and is implicated in Alzheimer's disease (AD) and frontotemporal lobar degeneration (FTLD-TDP) with progranulin (PGRN) deficiency. Here, we show that PGRN is involved in lysosomal homeostasis and lipid metabolism. PGRN deficiency alters lysosome abundance and morphology in mouse neurons. Using an unbiased lipidomic approach, we found that brain lipid composition in humans and mice with PGRN deficiency shows disease-specific differences that distinguish them from normal and other pathologic groups. PGRN loss leads to an accumulation of polyunsaturated triacylglycerides, as well as a reduction of diacylglycerides and phosphatidylserines in fibroblast and enriched lysosome lipidomes. Transcriptomic analysis of PGRN-deficient mouse brains revealed distinct expression patterns of lysosomal, immune-related, and lipid metabolic genes. These findings have implications for the pathogenesis of FTLD-TDP due to PGRN deficiency and suggest lysosomal dysfunction as an underlying mechanism.

Original languageEnglish (US)
Pages (from-to)2565-2574
Number of pages10
JournalCell Reports
Volume20
Issue number11
DOIs
StatePublished - Sep 12 2017

Fingerprint

Frontotemporal Dementia
Brain
Ceroid
Neurodegenerative diseases
Lysosomes
Lipids
Phosphatidylserines
Fibroblasts
Frontotemporal Lobar Degeneration
Neuronal Ceroid-Lipofuscinoses
Neurons
Deficiency Diseases
Genes
Lipid Metabolism
Neurodegenerative Diseases
Alzheimer Disease
Homeostasis
Chemical analysis

Keywords

  • Alzheimer's disease
  • frontotemporal lobar degeneration
  • lipidomics
  • lipids
  • lysosomal storage disorders
  • lysosome
  • neurodegeneration
  • neuronal ceroid lipofuscinoses
  • transcriptomics

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Lipidomic and Transcriptomic Basis of Lysosomal Dysfunction in Progranulin Deficiency. / Evers, Bret M.; Rodriguez-Navas, Carlos; Tesla, Rachel J.; Prange-Kiel, Janine; Wasser, Catherine R.; Yoo, Kyoung Shin; McDonald, Jeffrey; Cenik, Basar; Ravenscroft, Thomas A.; Plattner, Florian; Rademakers, Rosa; Yu, Gang; White, Charles L.; Herz, Joachim.

In: Cell Reports, Vol. 20, No. 11, 12.09.2017, p. 2565-2574.

Research output: Contribution to journalArticle

Evers, BM, Rodriguez-Navas, C, Tesla, RJ, Prange-Kiel, J, Wasser, CR, Yoo, KS, McDonald, J, Cenik, B, Ravenscroft, TA, Plattner, F, Rademakers, R, Yu, G, White, CL & Herz, J 2017, 'Lipidomic and Transcriptomic Basis of Lysosomal Dysfunction in Progranulin Deficiency', Cell Reports, vol. 20, no. 11, pp. 2565-2574. https://doi.org/10.1016/j.celrep.2017.08.056
Evers, Bret M. ; Rodriguez-Navas, Carlos ; Tesla, Rachel J. ; Prange-Kiel, Janine ; Wasser, Catherine R. ; Yoo, Kyoung Shin ; McDonald, Jeffrey ; Cenik, Basar ; Ravenscroft, Thomas A. ; Plattner, Florian ; Rademakers, Rosa ; Yu, Gang ; White, Charles L. ; Herz, Joachim. / Lipidomic and Transcriptomic Basis of Lysosomal Dysfunction in Progranulin Deficiency. In: Cell Reports. 2017 ; Vol. 20, No. 11. pp. 2565-2574.
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