Lipidomics reveals a remarkable diversity of lipids in human plasma1

Oswald Quehenberger, Aaron M. Armando, Alex H. Brown, Stephen B. Milne, David S. Myers, Alfred H. Merrill, Sibali Bandyopadhyay, Kristin N. Jones, Samuel Kelly, Rebecca L. Shaner, Cameron M. Sullards, Elaine Wang, Robert C. Murphy, Robert M. Barkley, Thomas J. Leiker, Christian R H Raetz, Ziqiang Guan, Gregory M. Laird, David A. Six, David W. Russell & 4 others Jeffrey G. McDonald, Shankar Subramaniam, Eoin Fahy, Edward A. Dennis

Research output: Contribution to journalArticle

589 Citations (Scopus)

Abstract

The focus of the present study was to define the human plasma lipidome and to establish novel analytical methodologies to quantify the large spectrum of plasma lipids. Partial lipid analysis is now a regular part of every patient's blood test and physicians readily and regularly prescribe drugs that alter the levels of major plasma lipids such as cholesterol and triglycerides. Plasma contains many thousands of distinct lipid molecular species that fall into six main categories including fatty acyls, glycerolipids, glycerophospholipids, sphingolipids, sterols, and prenols. The physiological contributions of these diverse lipids and how their levels change in response to therapy remain largely unknown. As a first step toward answering these questions, we provide herein an in-depth lipidomics analysis of a pooled human plasma obtained from healthy individuals after overnight fasting and with a gender balance and an ethnic distribution that is representative of the US population. In total, we quantitatively assessed the levels of over 500 distinct molecular species distributed among the main lipid categories. As more information is obtained regarding the roles of individual lipids in health and disease, it seems likely that future blood tests will include an ever increasing number of these lipid molecules.

Original languageEnglish (US)
Pages (from-to)3299-3305
Number of pages7
JournalJournal of Lipid Research
Volume51
Issue number11
DOIs
StatePublished - Nov 2010

Fingerprint

Lipids
Plasma (human)
Hematologic Tests
Plasmas
Blood
Glycerophospholipids
Sphingolipids
Sterols
Fasting
Triglycerides
Cholesterol
Health
Physicians
Molecules
Pharmaceutical Preparations
Population

Keywords

  • Human lipidome
  • Mass spectrometry
  • Metabolomics
  • Reference material

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Endocrinology

Cite this

Quehenberger, O., Armando, A. M., Brown, A. H., Milne, S. B., Myers, D. S., Merrill, A. H., ... Dennis, E. A. (2010). Lipidomics reveals a remarkable diversity of lipids in human plasma1. Journal of Lipid Research, 51(11), 3299-3305. https://doi.org/10.1194/jlr.M009449

Lipidomics reveals a remarkable diversity of lipids in human plasma1. / Quehenberger, Oswald; Armando, Aaron M.; Brown, Alex H.; Milne, Stephen B.; Myers, David S.; Merrill, Alfred H.; Bandyopadhyay, Sibali; Jones, Kristin N.; Kelly, Samuel; Shaner, Rebecca L.; Sullards, Cameron M.; Wang, Elaine; Murphy, Robert C.; Barkley, Robert M.; Leiker, Thomas J.; Raetz, Christian R H; Guan, Ziqiang; Laird, Gregory M.; Six, David A.; Russell, David W.; McDonald, Jeffrey G.; Subramaniam, Shankar; Fahy, Eoin; Dennis, Edward A.

In: Journal of Lipid Research, Vol. 51, No. 11, 11.2010, p. 3299-3305.

Research output: Contribution to journalArticle

Quehenberger, O, Armando, AM, Brown, AH, Milne, SB, Myers, DS, Merrill, AH, Bandyopadhyay, S, Jones, KN, Kelly, S, Shaner, RL, Sullards, CM, Wang, E, Murphy, RC, Barkley, RM, Leiker, TJ, Raetz, CRH, Guan, Z, Laird, GM, Six, DA, Russell, DW, McDonald, JG, Subramaniam, S, Fahy, E & Dennis, EA 2010, 'Lipidomics reveals a remarkable diversity of lipids in human plasma1', Journal of Lipid Research, vol. 51, no. 11, pp. 3299-3305. https://doi.org/10.1194/jlr.M009449
Quehenberger O, Armando AM, Brown AH, Milne SB, Myers DS, Merrill AH et al. Lipidomics reveals a remarkable diversity of lipids in human plasma1. Journal of Lipid Research. 2010 Nov;51(11):3299-3305. https://doi.org/10.1194/jlr.M009449
Quehenberger, Oswald ; Armando, Aaron M. ; Brown, Alex H. ; Milne, Stephen B. ; Myers, David S. ; Merrill, Alfred H. ; Bandyopadhyay, Sibali ; Jones, Kristin N. ; Kelly, Samuel ; Shaner, Rebecca L. ; Sullards, Cameron M. ; Wang, Elaine ; Murphy, Robert C. ; Barkley, Robert M. ; Leiker, Thomas J. ; Raetz, Christian R H ; Guan, Ziqiang ; Laird, Gregory M. ; Six, David A. ; Russell, David W. ; McDonald, Jeffrey G. ; Subramaniam, Shankar ; Fahy, Eoin ; Dennis, Edward A. / Lipidomics reveals a remarkable diversity of lipids in human plasma1. In: Journal of Lipid Research. 2010 ; Vol. 51, No. 11. pp. 3299-3305.
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