Lipopolysaccharide-treated raw 264.7 cells produce a mediator that inhibits lipoprotein lipase in 3T3-L1 cells

J. R. Mahoney, B. A. Beutler, N. Le Trang, W. Vine, Y. Ikeda, M. Kawakami, A. Cerami

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

RAW 264.7 cells upon stimulation with lipopolysaccharide secrete a protein mediator(s) that suppresses lipoprotein lipase activity in differentiated 3T3-L1 cells. The mediator(s), which is absent from unstimulated culture supernatants, is nondialyzable and thermolabile. Preliminary characterization suggests that this mediator(s) may be the same as that previously found in medium from lipopolysaccharide-treated thioglycollate-elicited mouse peritoneal macrophage cultures.

Original languageEnglish (US)
Pages (from-to)1673-1675
Number of pages3
JournalJournal of Immunology
Volume134
Issue number3
StatePublished - 1985

Fingerprint

3T3-L1 Cells
Lipoprotein Lipase
Lipopolysaccharides
Thioglycolates
Peritoneal Macrophages
Proteins
RAW 264.7 Cells

ASJC Scopus subject areas

  • Immunology

Cite this

Mahoney, J. R., Beutler, B. A., Le Trang, N., Vine, W., Ikeda, Y., Kawakami, M., & Cerami, A. (1985). Lipopolysaccharide-treated raw 264.7 cells produce a mediator that inhibits lipoprotein lipase in 3T3-L1 cells. Journal of Immunology, 134(3), 1673-1675.

Lipopolysaccharide-treated raw 264.7 cells produce a mediator that inhibits lipoprotein lipase in 3T3-L1 cells. / Mahoney, J. R.; Beutler, B. A.; Le Trang, N.; Vine, W.; Ikeda, Y.; Kawakami, M.; Cerami, A.

In: Journal of Immunology, Vol. 134, No. 3, 1985, p. 1673-1675.

Research output: Contribution to journalArticle

Mahoney, JR, Beutler, BA, Le Trang, N, Vine, W, Ikeda, Y, Kawakami, M & Cerami, A 1985, 'Lipopolysaccharide-treated raw 264.7 cells produce a mediator that inhibits lipoprotein lipase in 3T3-L1 cells', Journal of Immunology, vol. 134, no. 3, pp. 1673-1675.
Mahoney, J. R. ; Beutler, B. A. ; Le Trang, N. ; Vine, W. ; Ikeda, Y. ; Kawakami, M. ; Cerami, A. / Lipopolysaccharide-treated raw 264.7 cells produce a mediator that inhibits lipoprotein lipase in 3T3-L1 cells. In: Journal of Immunology. 1985 ; Vol. 134, No. 3. pp. 1673-1675.
@article{ff051caeb65740d699dd763db0c86226,
title = "Lipopolysaccharide-treated raw 264.7 cells produce a mediator that inhibits lipoprotein lipase in 3T3-L1 cells",
abstract = "RAW 264.7 cells upon stimulation with lipopolysaccharide secrete a protein mediator(s) that suppresses lipoprotein lipase activity in differentiated 3T3-L1 cells. The mediator(s), which is absent from unstimulated culture supernatants, is nondialyzable and thermolabile. Preliminary characterization suggests that this mediator(s) may be the same as that previously found in medium from lipopolysaccharide-treated thioglycollate-elicited mouse peritoneal macrophage cultures.",
author = "Mahoney, {J. R.} and Beutler, {B. A.} and {Le Trang}, N. and W. Vine and Y. Ikeda and M. Kawakami and A. Cerami",
year = "1985",
language = "English (US)",
volume = "134",
pages = "1673--1675",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "3",

}

TY - JOUR

T1 - Lipopolysaccharide-treated raw 264.7 cells produce a mediator that inhibits lipoprotein lipase in 3T3-L1 cells

AU - Mahoney, J. R.

AU - Beutler, B. A.

AU - Le Trang, N.

AU - Vine, W.

AU - Ikeda, Y.

AU - Kawakami, M.

AU - Cerami, A.

PY - 1985

Y1 - 1985

N2 - RAW 264.7 cells upon stimulation with lipopolysaccharide secrete a protein mediator(s) that suppresses lipoprotein lipase activity in differentiated 3T3-L1 cells. The mediator(s), which is absent from unstimulated culture supernatants, is nondialyzable and thermolabile. Preliminary characterization suggests that this mediator(s) may be the same as that previously found in medium from lipopolysaccharide-treated thioglycollate-elicited mouse peritoneal macrophage cultures.

AB - RAW 264.7 cells upon stimulation with lipopolysaccharide secrete a protein mediator(s) that suppresses lipoprotein lipase activity in differentiated 3T3-L1 cells. The mediator(s), which is absent from unstimulated culture supernatants, is nondialyzable and thermolabile. Preliminary characterization suggests that this mediator(s) may be the same as that previously found in medium from lipopolysaccharide-treated thioglycollate-elicited mouse peritoneal macrophage cultures.

UR - http://www.scopus.com/inward/record.url?scp=0021988474&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021988474&partnerID=8YFLogxK

M3 - Article

C2 - 3968430

AN - SCOPUS:0021988474

VL - 134

SP - 1673

EP - 1675

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 3

ER -