Lipoproteins of Haemophilus influenzae type b

G. A. Weinberg, D. A. Towler, R. S. Munson

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Haemophilus influenzae type b Minn A produced 12 lipoproteins with apparent molecular weights of between 14,000 adn 67,000. The lipoproteins were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography of delipidated extracts of cells grown in [3H]palmitate. When the delipidated cell extracts were subjected to acid methanolysis, tritium was quantitatively recovered as palmitate and methyl palmitate, indicating that the [3H]palmitate had not been degraded and reincorporated into nonlipid material during cell growth. One of the lipoproteins comigrated with outer membrane protein (OMP) P6. OMP P6 was purified from [3H]palmitate-labeled cells. The purified protein preparation contained both amide- and ester-linked fatty acids. We conclude that (i) H. influenzae type b produces several lipoproteins, and (ii) one of these lipoproteins is OMP P6, a protein under consideration as a vaccine component.

Original languageEnglish (US)
Pages (from-to)4161-4164
Number of pages4
JournalJournal of bacteriology
Volume170
Issue number9
DOIs
StatePublished - 1988

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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