Lipoproteins of Haemophilus influenzae type b

G. A. Weinberg, D. A. Towler, R. S. Munson

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Haemophilus influenzae type b Minn A produced 12 lipoproteins with apparent molecular weights of between 14,000 adn 67,000. The lipoproteins were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography of delipidated extracts of cells grown in [3H]palmitate. When the delipidated cell extracts were subjected to acid methanolysis, tritium was quantitatively recovered as palmitate and methyl palmitate, indicating that the [3H]palmitate had not been degraded and reincorporated into nonlipid material during cell growth. One of the lipoproteins comigrated with outer membrane protein (OMP) P6. OMP P6 was purified from [3H]palmitate-labeled cells. The purified protein preparation contained both amide- and ester-linked fatty acids. We conclude that (i) H. influenzae type b produces several lipoproteins, and (ii) one of these lipoproteins is OMP P6, a protein under consideration as a vaccine component.

Original languageEnglish (US)
Pages (from-to)4161-4164
Number of pages4
JournalJournal of Bacteriology
Volume170
Issue number9
DOIs
StatePublished - Jan 1 1988

Fingerprint

Haemophilus influenzae type b
Palmitates
Lipoproteins
Membrane Proteins
Cell Extracts
Photofluorography
Tritium
Amides
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Esters
Proteins
Fatty Acids
Vaccines
Molecular Weight
Acids
Growth

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Lipoproteins of Haemophilus influenzae type b. / Weinberg, G. A.; Towler, D. A.; Munson, R. S.

In: Journal of Bacteriology, Vol. 170, No. 9, 01.01.1988, p. 4161-4164.

Research output: Contribution to journalArticle

Weinberg, G. A. ; Towler, D. A. ; Munson, R. S. / Lipoproteins of Haemophilus influenzae type b. In: Journal of Bacteriology. 1988 ; Vol. 170, No. 9. pp. 4161-4164.
@article{0775eeae800a4321a859b36f72caad4d,
title = "Lipoproteins of Haemophilus influenzae type b",
abstract = "Haemophilus influenzae type b Minn A produced 12 lipoproteins with apparent molecular weights of between 14,000 adn 67,000. The lipoproteins were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography of delipidated extracts of cells grown in [3H]palmitate. When the delipidated cell extracts were subjected to acid methanolysis, tritium was quantitatively recovered as palmitate and methyl palmitate, indicating that the [3H]palmitate had not been degraded and reincorporated into nonlipid material during cell growth. One of the lipoproteins comigrated with outer membrane protein (OMP) P6. OMP P6 was purified from [3H]palmitate-labeled cells. The purified protein preparation contained both amide- and ester-linked fatty acids. We conclude that (i) H. influenzae type b produces several lipoproteins, and (ii) one of these lipoproteins is OMP P6, a protein under consideration as a vaccine component.",
author = "Weinberg, {G. A.} and Towler, {D. A.} and Munson, {R. S.}",
year = "1988",
month = "1",
day = "1",
doi = "10.1128/jb.170.9.4161-4164.1988",
language = "English (US)",
volume = "170",
pages = "4161--4164",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "9",

}

TY - JOUR

T1 - Lipoproteins of Haemophilus influenzae type b

AU - Weinberg, G. A.

AU - Towler, D. A.

AU - Munson, R. S.

PY - 1988/1/1

Y1 - 1988/1/1

N2 - Haemophilus influenzae type b Minn A produced 12 lipoproteins with apparent molecular weights of between 14,000 adn 67,000. The lipoproteins were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography of delipidated extracts of cells grown in [3H]palmitate. When the delipidated cell extracts were subjected to acid methanolysis, tritium was quantitatively recovered as palmitate and methyl palmitate, indicating that the [3H]palmitate had not been degraded and reincorporated into nonlipid material during cell growth. One of the lipoproteins comigrated with outer membrane protein (OMP) P6. OMP P6 was purified from [3H]palmitate-labeled cells. The purified protein preparation contained both amide- and ester-linked fatty acids. We conclude that (i) H. influenzae type b produces several lipoproteins, and (ii) one of these lipoproteins is OMP P6, a protein under consideration as a vaccine component.

AB - Haemophilus influenzae type b Minn A produced 12 lipoproteins with apparent molecular weights of between 14,000 adn 67,000. The lipoproteins were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography of delipidated extracts of cells grown in [3H]palmitate. When the delipidated cell extracts were subjected to acid methanolysis, tritium was quantitatively recovered as palmitate and methyl palmitate, indicating that the [3H]palmitate had not been degraded and reincorporated into nonlipid material during cell growth. One of the lipoproteins comigrated with outer membrane protein (OMP) P6. OMP P6 was purified from [3H]palmitate-labeled cells. The purified protein preparation contained both amide- and ester-linked fatty acids. We conclude that (i) H. influenzae type b produces several lipoproteins, and (ii) one of these lipoproteins is OMP P6, a protein under consideration as a vaccine component.

UR - http://www.scopus.com/inward/record.url?scp=0023780696&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023780696&partnerID=8YFLogxK

U2 - 10.1128/jb.170.9.4161-4164.1988

DO - 10.1128/jb.170.9.4161-4164.1988

M3 - Article

C2 - 3261724

AN - SCOPUS:0023780696

VL - 170

SP - 4161

EP - 4164

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 9

ER -