Liquid chromatography-mass spectrometric analysis of lipids present in human meibomian gland secretions

Igor Butovich, Eduardo Uchiyama, Mario A. Di Pascuale, James P McCulley

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

The purpose of the study was to qualitatively characterize the major lipid species present in human meibomian gland secretions (MGS) by means of high-performance liquid chromatography with atmospheric pressure ionization mass spectrometric detection of the analytes (NP HPLC-MS). Two different NP HPLC-MS methods have been developed to analyze lipid species that were expected to be present in MGS. The first method was optimized for the analysis of relatively nonpolar lipids [wax esters (WE), di- and triacyl glycerols (DAG and TAG), cholesterol (Chl) and its esters (Chl-E), and ceramides (Cer)], while the second method was designed to separate and detect phospholipids. The major lipid species in MGS were found to be WE, Chl-E, and TAG. A minor amount of free Chi (less then 0.5% of the Chl-E fraction) was detected in MGS. No appreciable amounts of DAG and Cer were found in MGS. The second NP HPLC-MS method, capable of analyzing model mixtures of authentic phospholipids (e.g. phosphatidylglycerol, phosphatidylethanolamine, phosphatidic acid, phosphatidylinositol, phosphatidylserine, phosphatidylcholine, and sphingomyelin) in submicrogram/mL concentrations, showed little or no presence of these species in the MGS samples. These observations suggest that MGS are a major source of the nonpolar lipids of the WE and Chl-E families for the tear film lipid layer (TFLL), but not of the previously reported phospholipid components of the TFLL.

Original languageEnglish (US)
Pages (from-to)765-776
Number of pages12
JournalLipids
Volume42
Issue number8
DOIs
StatePublished - Aug 2007

Fingerprint

Meibomian Glands
Liquid chromatography
Liquid Chromatography
liquid chromatography
secretion
Lipids
wax esters
lipids
Cholesterol Esters
Waxes
high performance liquid chromatography
High Pressure Liquid Chromatography
cholesterol
Phospholipids
phospholipids
ceramides
Ceramides
Tears
films (materials)
sphingomyelins

Keywords

  • HPLC
  • Human meibomian gland
  • Lipids
  • Mass spectrometry
  • Tear film lipid layer

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Food Science
  • Biochemistry

Cite this

Liquid chromatography-mass spectrometric analysis of lipids present in human meibomian gland secretions. / Butovich, Igor; Uchiyama, Eduardo; Di Pascuale, Mario A.; McCulley, James P.

In: Lipids, Vol. 42, No. 8, 08.2007, p. 765-776.

Research output: Contribution to journalArticle

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