Listeria monocytogenes-infected hepatocytes are targets of major histocompatibility complex class Ib-restricted antilisterial cytotoxic T lymphocytes

H. G.Archie Bouwer, Ailin Bai, James Forman, Stephen H. Gregory, Edward J. Wing, Ronald A. Barry, David J. Hinrichs

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Subclinical infection of BALB/c mice with the intracellular bacterial pathogen Listeria monocytogenes results in the development of protective antilisterial immunity. L. monocytogenes can infect hepatocytes, and antilisterial cytotoxic T lymphocytes (CTL) lyse Listeria-infected hepatocytes in a major histocompatibility complex (MHC) class Ia-restricted manner. It remained to be determined whether L. monocytogenes-infected hepatocytes are susceptible to MHC class Ib-restricted cytolysis. In this study, we showed that hepatocytes express MHC class Ib molecule Qa-1(b) mRNA and protein. We further showed that Listeria-infected hepatocytes are susceptible to MHC class Ib-restricted cytolysis, since C57BL/6-derived Listeria-infected hepatocytes were lysed by BALB/c-derived antilisterial CTL. These results establish that Listeria-infected hepatocytes are susceptible to cytolysis by MHC class lb restricted Listeria-specific CTL.

Original languageEnglish (US)
Pages (from-to)2814-2817
Number of pages4
JournalInfection and Immunity
Volume66
Issue number6
StatePublished - Jun 1 1998

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Listeria monocytogenes
Cytotoxic T-Lymphocytes
Major Histocompatibility Complex
Listeria
Hepatocytes
Asymptomatic Infections
Immunity
Messenger RNA
Proteins

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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Listeria monocytogenes-infected hepatocytes are targets of major histocompatibility complex class Ib-restricted antilisterial cytotoxic T lymphocytes. / Bouwer, H. G.Archie; Bai, Ailin; Forman, James; Gregory, Stephen H.; Wing, Edward J.; Barry, Ronald A.; Hinrichs, David J.

In: Infection and Immunity, Vol. 66, No. 6, 01.06.1998, p. 2814-2817.

Research output: Contribution to journalArticle

Bouwer, H. G.Archie ; Bai, Ailin ; Forman, James ; Gregory, Stephen H. ; Wing, Edward J. ; Barry, Ronald A. ; Hinrichs, David J. / Listeria monocytogenes-infected hepatocytes are targets of major histocompatibility complex class Ib-restricted antilisterial cytotoxic T lymphocytes. In: Infection and Immunity. 1998 ; Vol. 66, No. 6. pp. 2814-2817.
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abstract = "Subclinical infection of BALB/c mice with the intracellular bacterial pathogen Listeria monocytogenes results in the development of protective antilisterial immunity. L. monocytogenes can infect hepatocytes, and antilisterial cytotoxic T lymphocytes (CTL) lyse Listeria-infected hepatocytes in a major histocompatibility complex (MHC) class Ia-restricted manner. It remained to be determined whether L. monocytogenes-infected hepatocytes are susceptible to MHC class Ib-restricted cytolysis. In this study, we showed that hepatocytes express MHC class Ib molecule Qa-1(b) mRNA and protein. We further showed that Listeria-infected hepatocytes are susceptible to MHC class Ib-restricted cytolysis, since C57BL/6-derived Listeria-infected hepatocytes were lysed by BALB/c-derived antilisterial CTL. These results establish that Listeria-infected hepatocytes are susceptible to cytolysis by MHC class lb restricted Listeria-specific CTL.",
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AU - Forman, James

AU - Gregory, Stephen H.

AU - Wing, Edward J.

AU - Barry, Ronald A.

AU - Hinrichs, David J.

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AB - Subclinical infection of BALB/c mice with the intracellular bacterial pathogen Listeria monocytogenes results in the development of protective antilisterial immunity. L. monocytogenes can infect hepatocytes, and antilisterial cytotoxic T lymphocytes (CTL) lyse Listeria-infected hepatocytes in a major histocompatibility complex (MHC) class Ia-restricted manner. It remained to be determined whether L. monocytogenes-infected hepatocytes are susceptible to MHC class Ib-restricted cytolysis. In this study, we showed that hepatocytes express MHC class Ib molecule Qa-1(b) mRNA and protein. We further showed that Listeria-infected hepatocytes are susceptible to MHC class Ib-restricted cytolysis, since C57BL/6-derived Listeria-infected hepatocytes were lysed by BALB/c-derived antilisterial CTL. These results establish that Listeria-infected hepatocytes are susceptible to cytolysis by MHC class lb restricted Listeria-specific CTL.

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