@inbook{e7699838c69d47ad905b685440c83705,
title = "Live-cell imaging of the adult drosophila ovary using confocal microscopy",
abstract = "The Drosophila ovary represents a key in vivo model used to study germline stem cell (GSC) maintenance and stem cell daughter differentiation because these cells and their somatic cell neighbors can be identified at single-cell resolution within their native environment. Here we describe a fluorescent-based technique for the acquisition of 4D datasets of the Drosophila ovariole for periods that can exceed 12 consecutive hours. Live-cell imaging facilitates the investigation of molecular and cellular dynamics that were not previously possible using still images.",
keywords = "Cellular dynamics, Confocal microscopy, Drosophila ovary, Live-cell imaging, Molecular dynamics",
author = "Shalaby, {Nevine A.} and Michael Buszczak",
year = "2017",
doi = "10.1007/978-1-4939-4017-2_6",
language = "English (US)",
volume = "1463",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "85--91",
booktitle = "Methods in Molecular Biology",
}