Live-cell imaging of the adult drosophila ovary using confocal microscopy

Nevine A. Shalaby, Michael Buszczak

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

The Drosophila ovary represents a key in vivo model used to study germline stem cell (GSC) maintenance and stem cell daughter differentiation because these cells and their somatic cell neighbors can be identified at single-cell resolution within their native environment. Here we describe a fluorescent-based technique for the acquisition of 4D datasets of the Drosophila ovariole for periods that can exceed 12 consecutive hours. Live-cell imaging facilitates the investigation of molecular and cellular dynamics that were not previously possible using still images.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages85-91
Number of pages7
Volume1463
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1463
ISSN (Print)10643745

Keywords

  • Cellular dynamics
  • Confocal microscopy
  • Drosophila ovary
  • Live-cell imaging
  • Molecular dynamics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Shalaby, N. A., & Buszczak, M. (2017). Live-cell imaging of the adult drosophila ovary using confocal microscopy. In Methods in Molecular Biology (Vol. 1463, pp. 85-91). (Methods in Molecular Biology; Vol. 1463). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-4017-2_6