Localization and phosphorylation of AbI-interactor proteins, Abi-1 and Abi-2, in the developing nervous system

Kevin D. Courtney, Matthew Grove, Hendrika Vandongen, Antonius Vandongen, Anthony Samuel LaMantia, Ann Marie Pendergast

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

AbI-interactor (Abi) proteins are targets of AbI-family nonreceptor tyrosine kinases and are required for Rac-dependent cytoskeletal reorganization in response to growth factor stimulation. We asked if the expression, phosphorylation, and cellular localization of Abi-1 and Abi-2 supports a role for these proteins in AbI signaling in the developing and adult mouse nervous system. In mid to late-gestation embryos, abi-2 message is elevated in the central and peripheral nervous systems (CNS and PNS). Abi-l mRNA is present, but not enhanced, in the CNS, and is not observed in PNS structures. Abi proteins from brain iysates undergo changes in apparent molecular weight and phosphorylation with increasing age. In the postnatal brain, abi-1 and abi-2 are expressed most prominently in cortical layers populated by projection neurons. In cultured neurons, Abi-1 and Abi-2 are concentrated in puncta throughout the cell body and processes. Both Abi and AbI proteins are present in synaptosomes and growth cone particles. Therefore, the Abi adaptors exhibit proper expression patterns and subcellular localization to participate in AbI kinase signaling in the nervous system.

Original languageEnglish (US)
Pages (from-to)244-257
Number of pages14
JournalMolecular and Cellular Neurosciences
Volume16
Issue number3
DOIs
StatePublished - 2000

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Nervous System
Phosphorylation
Proteins
Neurons
Growth Cones
Synaptosomes
Peripheral Nervous System
Brain
Protein-Tyrosine Kinases
Intercellular Signaling Peptides and Proteins
Phosphotransferases
Embryonic Structures
Central Nervous System
Molecular Weight
Pregnancy
Messenger RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Localization and phosphorylation of AbI-interactor proteins, Abi-1 and Abi-2, in the developing nervous system. / Courtney, Kevin D.; Grove, Matthew; Vandongen, Hendrika; Vandongen, Antonius; LaMantia, Anthony Samuel; Pendergast, Ann Marie.

In: Molecular and Cellular Neurosciences, Vol. 16, No. 3, 2000, p. 244-257.

Research output: Contribution to journalArticle

Courtney, Kevin D. ; Grove, Matthew ; Vandongen, Hendrika ; Vandongen, Antonius ; LaMantia, Anthony Samuel ; Pendergast, Ann Marie. / Localization and phosphorylation of AbI-interactor proteins, Abi-1 and Abi-2, in the developing nervous system. In: Molecular and Cellular Neurosciences. 2000 ; Vol. 16, No. 3. pp. 244-257.
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