TY - JOUR
T1 - Localization and phosphorylation of AbI-interactor proteins, Abi-1 and Abi-2, in the developing nervous system
AU - Courtney, Kevin D.
AU - Grove, Matthew
AU - Vandongen, Hendrika
AU - Vandongen, Antonius
AU - LaMantia, Anthony Samuel
AU - Pendergast, Ann Marie
N1 - Funding Information:
We are grateful to Dr. R. T. Fremeau for generously providing technical guidance and reagents. We thank Dr. N. Cant for technical assistance with microscopy and image preparation and Dr. J. H. P. Skene, Dr. R. J. Colbran, and Dr. A. J. Koleske for kindly providing reagents. We thank Dr. A. R. Means, Dr. P. A. Zipfel, and Dr. R. Plattner for reviewing the manuscript. This work was supported by National Cancer Institute grant CA70940 (A.M.P.). K.D.C. was supported by the Medical Scientist Training Program and the Department of Defense Breast Cancer Research Program. A.M.P. is a Scholar of the Leukemia Society of America.
PY - 2000
Y1 - 2000
N2 - AbI-interactor (Abi) proteins are targets of AbI-family nonreceptor tyrosine kinases and are required for Rac-dependent cytoskeletal reorganization in response to growth factor stimulation. We asked if the expression, phosphorylation, and cellular localization of Abi-1 and Abi-2 supports a role for these proteins in AbI signaling in the developing and adult mouse nervous system. In mid to late-gestation embryos, abi-2 message is elevated in the central and peripheral nervous systems (CNS and PNS). Abi-l mRNA is present, but not enhanced, in the CNS, and is not observed in PNS structures. Abi proteins from brain iysates undergo changes in apparent molecular weight and phosphorylation with increasing age. In the postnatal brain, abi-1 and abi-2 are expressed most prominently in cortical layers populated by projection neurons. In cultured neurons, Abi-1 and Abi-2 are concentrated in puncta throughout the cell body and processes. Both Abi and AbI proteins are present in synaptosomes and growth cone particles. Therefore, the Abi adaptors exhibit proper expression patterns and subcellular localization to participate in AbI kinase signaling in the nervous system.
AB - AbI-interactor (Abi) proteins are targets of AbI-family nonreceptor tyrosine kinases and are required for Rac-dependent cytoskeletal reorganization in response to growth factor stimulation. We asked if the expression, phosphorylation, and cellular localization of Abi-1 and Abi-2 supports a role for these proteins in AbI signaling in the developing and adult mouse nervous system. In mid to late-gestation embryos, abi-2 message is elevated in the central and peripheral nervous systems (CNS and PNS). Abi-l mRNA is present, but not enhanced, in the CNS, and is not observed in PNS structures. Abi proteins from brain iysates undergo changes in apparent molecular weight and phosphorylation with increasing age. In the postnatal brain, abi-1 and abi-2 are expressed most prominently in cortical layers populated by projection neurons. In cultured neurons, Abi-1 and Abi-2 are concentrated in puncta throughout the cell body and processes. Both Abi and AbI proteins are present in synaptosomes and growth cone particles. Therefore, the Abi adaptors exhibit proper expression patterns and subcellular localization to participate in AbI kinase signaling in the nervous system.
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U2 - 10.1006/mcne.2000.0865
DO - 10.1006/mcne.2000.0865
M3 - Article
C2 - 10995551
AN - SCOPUS:0033623190
SN - 1044-7431
VL - 16
SP - 244
EP - 257
JO - Molecular and Cellular Neurosciences
JF - Molecular and Cellular Neurosciences
IS - 3
ER -