Localization of an actin binding domain in smooth muscle myosin light chain kinase

Patricia J. Gallagher, James T. Stull

Research output: Contribution to journalArticle

24 Scopus citations


Phosphorylation of the regulatory light chain of myosin II by myosin light chain kinase is important for regulating many contractile processes. Smooth muscle myosin light chain kinase has been shown to be associated with both actin and myosin filaments in vitro and in vivo. In this report we define an actin binding region by using molecular deletions to generate recombinant mutant proteins that were analyzed by co-sedimentation with F-actin. An actin binding region restricted to residues 2-42 in the animo terminus of the rabbit smooth muscle myosin light chain kinase was identified.

Original languageEnglish (US)
Pages (from-to)51-57
Number of pages7
JournalMolecular and Cellular Biochemistry
Issue number1-2
StatePublished - Sep 25 1997



  • Actin binding
  • Contraction
  • Myosin light chain kinase
  • Protein kinase

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

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