Localization of laminin isoforms in the guinea pig cochlea

Objective: To elucidate the pathogenesis of inner ear disorders by examining the distribution of a major component of basement membranes, the glycoprotein laminin. Study Design: Animal model. Methods: Adult guinea pig cochleae were fixed with methanol and sectioned. Sections were examined for the presence of laminins α2, α5, β1, and γ1 using immunohistochemistry. Results: Laminins α2, α5, and β1 were not detected. Laminin γ1 was found in the limbus, spiral ligament, and stria vascularis. Conclusions: Anti- laminin antibodies have been found in patients with inner ear disorders. Laminin γ1 demonstrates specific staining at multiple sites in the guinea pig cochlea. These structures may be targets of antibodies causing sensorineural hearing loss.