Localization of laminin isoforms in the guinea pig cochlea

Debra G. Weinberger, Wouter Jan Ten Cate, Juergen Lautermann, Martina Baethmann

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objective: To elucidate the pathogenesis of inner ear disorders by examining the distribution of a major component of basement membranes, the glycoprotein laminin. Study Design: Animal model. Methods: Adult guinea pig cochleae were fixed with methanol and sectioned. Sections were examined for the presence of laminins α2, α5, β1, and γ1 using immunohistochemistry. Results: Laminins α2, α5, and β1 were not detected. Laminin γ1 was found in the limbus, spiral ligament, and stria vascularis. Conclusions: Anti- laminin antibodies have been found in patients with inner ear disorders. Laminin γ1 demonstrates specific staining at multiple sites in the guinea pig cochlea. These structures may be targets of antibodies causing sensorineural hearing loss.

Original languageEnglish (US)
Pages (from-to)2001-2004
Number of pages4
JournalLaryngoscope
Volume109
Issue number12
StatePublished - Dec 1999

Fingerprint

Cochlea
Laminin
Protein Isoforms
Guinea Pigs
Inner Ear
Spiral Ligament of Cochlea
Stria Vascularis
Sensorineural Hearing Loss
Membrane Glycoproteins
Basement Membrane
Methanol
Anti-Idiotypic Antibodies
Animal Models
Immunohistochemistry
Staining and Labeling
Antibodies
laminin 1
kalinin

Keywords

  • Laminin
  • Sensorineural hearing loss

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Weinberger, D. G., Ten Cate, W. J., Lautermann, J., & Baethmann, M. (1999). Localization of laminin isoforms in the guinea pig cochlea. Laryngoscope, 109(12), 2001-2004.

Localization of laminin isoforms in the guinea pig cochlea. / Weinberger, Debra G.; Ten Cate, Wouter Jan; Lautermann, Juergen; Baethmann, Martina.

In: Laryngoscope, Vol. 109, No. 12, 12.1999, p. 2001-2004.

Research output: Contribution to journalArticle

Weinberger, DG, Ten Cate, WJ, Lautermann, J & Baethmann, M 1999, 'Localization of laminin isoforms in the guinea pig cochlea', Laryngoscope, vol. 109, no. 12, pp. 2001-2004.
Weinberger DG, Ten Cate WJ, Lautermann J, Baethmann M. Localization of laminin isoforms in the guinea pig cochlea. Laryngoscope. 1999 Dec;109(12):2001-2004.
Weinberger, Debra G. ; Ten Cate, Wouter Jan ; Lautermann, Juergen ; Baethmann, Martina. / Localization of laminin isoforms in the guinea pig cochlea. In: Laryngoscope. 1999 ; Vol. 109, No. 12. pp. 2001-2004.
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