Localization of nAChR subunit mRNAs in the brain of Macaca mulatta

Zhi Yan Han, Nicolas Le Novère, Michele Zoli, Joseph A Hill, Nicolas Champtiaux, Jean Pierre Changeux

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

We present here a systematic mapping of nAChR subunit mRNAs in Macaca mulatta brain. A fragment, from the transmembrane segments MIII to MIV of Macaca neuronal nAChR subunits was cloned, and shown to exhibit high identity (around 95%) to the corresponding human subunits. Then, specific oligodeoxynucleotides were synthesized for in situ hybridization experiments. Both α4 and β2 mRNA signals were widely distributed in the brain, being stronger in the thalamus and in the dopaminergic cells of the mesencephalon. Most brain nuclei displayed both α4 and β2 signals with the exception of some basal ganglia regions and the reticular thalamic nucleus which were devoid of α4 signal. α6 and β3 mRNA signals were selectively concentrated in the substantia nigra and the medial habenula. The strongest signals for α3 or β4 mRNAs were found in the epithalamus (medial habenula and pineal gland), whereas there were no specific α3 or β4 signals in mesencephalic dopaminergic nuclei. α5 and α7 mRNA signals were found in several brain areas, including cerebral cortex, thalamus and substantia nigra, although at a lower level than α4 and β2. The distribution of α3, α4, α5, α6, α7, β2, β3 and β4 subunit mRNAs in the monkey is substantially similar to that observed in rodent brain. Surprisingly, α2 mRNA signal was largely distributed in the Macaca brain, at levels comparable with those of α4 and β2. This observation represents the main difference between rodent and Macaca subunit mRNA distribution and suggests that, besides α4β2*, α2β2* nAChRs constitute a main nAChR isoform in primate brain.

Original languageEnglish (US)
Pages (from-to)3664-3674
Number of pages11
JournalEuropean Journal of Neuroscience
Volume12
Issue number10
DOIs
StatePublished - 2000

Fingerprint

Macaca mulatta
Messenger RNA
Brain
Macaca
Habenula
Substantia Nigra
Thalamus
Rodentia
Epithalamus
Thalamic Nuclei
Pineal Gland
Oligodeoxyribonucleotides
Mesencephalon
Basal Ganglia
Cerebral Cortex
Primates
Haplorhini
In Situ Hybridization
Protein Isoforms

Keywords

  • In situ hybridization
  • Neuroanatomy
  • Nicotinic acetylcholine receptors
  • Oligodeoxynucleotide probe
  • Primate

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Localization of nAChR subunit mRNAs in the brain of Macaca mulatta. / Han, Zhi Yan; Le Novère, Nicolas; Zoli, Michele; Hill, Joseph A; Champtiaux, Nicolas; Changeux, Jean Pierre.

In: European Journal of Neuroscience, Vol. 12, No. 10, 2000, p. 3664-3674.

Research output: Contribution to journalArticle

Han, ZY, Le Novère, N, Zoli, M, Hill, JA, Champtiaux, N & Changeux, JP 2000, 'Localization of nAChR subunit mRNAs in the brain of Macaca mulatta', European Journal of Neuroscience, vol. 12, no. 10, pp. 3664-3674. https://doi.org/10.1046/j.1460-9568.2000.00262.x
Han, Zhi Yan ; Le Novère, Nicolas ; Zoli, Michele ; Hill, Joseph A ; Champtiaux, Nicolas ; Changeux, Jean Pierre. / Localization of nAChR subunit mRNAs in the brain of Macaca mulatta. In: European Journal of Neuroscience. 2000 ; Vol. 12, No. 10. pp. 3664-3674.
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AB - We present here a systematic mapping of nAChR subunit mRNAs in Macaca mulatta brain. A fragment, from the transmembrane segments MIII to MIV of Macaca neuronal nAChR subunits was cloned, and shown to exhibit high identity (around 95%) to the corresponding human subunits. Then, specific oligodeoxynucleotides were synthesized for in situ hybridization experiments. Both α4 and β2 mRNA signals were widely distributed in the brain, being stronger in the thalamus and in the dopaminergic cells of the mesencephalon. Most brain nuclei displayed both α4 and β2 signals with the exception of some basal ganglia regions and the reticular thalamic nucleus which were devoid of α4 signal. α6 and β3 mRNA signals were selectively concentrated in the substantia nigra and the medial habenula. The strongest signals for α3 or β4 mRNAs were found in the epithalamus (medial habenula and pineal gland), whereas there were no specific α3 or β4 signals in mesencephalic dopaminergic nuclei. α5 and α7 mRNA signals were found in several brain areas, including cerebral cortex, thalamus and substantia nigra, although at a lower level than α4 and β2. The distribution of α3, α4, α5, α6, α7, β2, β3 and β4 subunit mRNAs in the monkey is substantially similar to that observed in rodent brain. Surprisingly, α2 mRNA signal was largely distributed in the Macaca brain, at levels comparable with those of α4 and β2. This observation represents the main difference between rodent and Macaca subunit mRNA distribution and suggests that, besides α4β2*, α2β2* nAChRs constitute a main nAChR isoform in primate brain.

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