Localized irregularities in hemoglobin flow and oxygenation in calf muscle in patients with peripheral vascular disease detected with near-infrared spectrophotometry

Ursula Wolf, Martin Wolf, Jee H. Choi, Moshe Levi, Devasmita Choudhury, Sherri Hull, Daniel Coussirat, L. Adelina Paunescu, Larisa P. Safonova, Antonios Michalos, William W. Mantulin, Enrico Gratton

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Purpose: Near-infrared spectrophotometry is used to measure flow, concentration, and oxygenation of hemoglobin in arterioles, capillaries, and venules several centimeters deep in tissue. The purpose of this study was to investigate the distribution of flow, concentration, and oxygenation of hemoglobin in calf muscle in patients with documented peripheral arterial occlusive disease (PVD), patients with risk factors for PVD, and healthy younger subjects at rest. Method: With a frequency-domain near-infrared spectrophotometer and a specially designed probe, we generated maps at 22 locations simultaneously of hemoglobin flow, concentration, and oxygenation, with the venous occlusion method. Eight legs of 7 patients with diagnosed PVD (PVD group), 10 legs of 8 patients with normal ankle-brachial index but with risk factors for PVD (RF group), and 16 legs of 8 healthy subjects (H group) were studied. Results: Global mean values were significantly (P < .05) different between the three groups for oxygen consumption (PVD group, 0.027 ± 0.009 mL/100 g/min; RF group, 0.038 ± 0.017 mL/100 g/min; H group, 0.022 ± 0.020 mL/100 g/min), venous oxygen saturation (PVD, 59.7% ± 15.4%; RF, 69.6% ± 10.5%; H, 80.8% ± 4.5%), and, at 60 s of venous occlusion, concentration changes in oxyhemoglobin (PVD, 4.48 ± 3.25 μmol/L; RF, 8.44 ± 2.33 μmol/L; H, 6.85 ± 4.57 μmol/L), deoxyhemoglobin (PVD, 3.60 ± 0.73 μmol/L; RF, 4.39 ± 1.30 μmol/L; H, 2.36 ± 1.79 μmol/L), and total hemoglobin (PVD, 8.07 ± 3.83 μmol/L; RF, 12.83 ± 2.75 μmol/L; H, 9.21 ± 6.34 μmol/L). No significant difference was found between the three groups for hemoglobin flow (PVD, 0.92 ± 0.69 μmol/100 mL/min; RF, 1.68 ± 0.50 μmol/100 mL/min; H, 1.44 ± 1.17 μmol/100 mL/min) and blood flow (PVD, 0.45 ± 0.28 mL/100 g/min; RF, 0.77 ± 0.21 mL/100 g/min; H, 0.62 ± 0.50 mL/100 g/min). All parameters featured a distribution dependent on location. Conclusion: Mean value for venous oxygen saturation was higher in healthy subjects compared to patients with documented PVD. In patients with PVD, areas of lower oxygenation were clearly discernible. At distal locations of calf muscle, significant correlations between reduced hemoglobin flow, venous oxygen saturation, oxyhemoglobin, and total hemoglobin and reduced ankle-brachial index were found. Maps revealed localized irregularities in oxyhemoglobin, total hemoglobin, and venous oxygen saturation in patients with PVD. Near-infrared spectrophotometry is a noninvasive bedside technique that can enable determination of blood flow and oxygenation in tissue and may provide a method for evaluating patients with PVD.

Original languageEnglish (US)
Pages (from-to)1017-1026
Number of pages10
JournalJournal of Vascular Surgery
Volume37
Issue number5
DOIs
StatePublished - May 1 2003

Fingerprint

Infrared Spectrophotometry
Peripheral Vascular Diseases
Hemoglobins
Muscles
Oxyhemoglobins
Oxygen
Ankle Brachial Index
Leg
Healthy Volunteers
Arterial Occlusive Diseases
Venules
Peripheral Arterial Disease
Arterioles
Oxygen Consumption

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

Cite this

Localized irregularities in hemoglobin flow and oxygenation in calf muscle in patients with peripheral vascular disease detected with near-infrared spectrophotometry. / Wolf, Ursula; Wolf, Martin; Choi, Jee H.; Levi, Moshe; Choudhury, Devasmita; Hull, Sherri; Coussirat, Daniel; Paunescu, L. Adelina; Safonova, Larisa P.; Michalos, Antonios; Mantulin, William W.; Gratton, Enrico.

In: Journal of Vascular Surgery, Vol. 37, No. 5, 01.05.2003, p. 1017-1026.

Research output: Contribution to journalArticle

Wolf, U, Wolf, M, Choi, JH, Levi, M, Choudhury, D, Hull, S, Coussirat, D, Paunescu, LA, Safonova, LP, Michalos, A, Mantulin, WW & Gratton, E 2003, 'Localized irregularities in hemoglobin flow and oxygenation in calf muscle in patients with peripheral vascular disease detected with near-infrared spectrophotometry', Journal of Vascular Surgery, vol. 37, no. 5, pp. 1017-1026. https://doi.org/10.1067/mva.2003.214
Wolf, Ursula ; Wolf, Martin ; Choi, Jee H. ; Levi, Moshe ; Choudhury, Devasmita ; Hull, Sherri ; Coussirat, Daniel ; Paunescu, L. Adelina ; Safonova, Larisa P. ; Michalos, Antonios ; Mantulin, William W. ; Gratton, Enrico. / Localized irregularities in hemoglobin flow and oxygenation in calf muscle in patients with peripheral vascular disease detected with near-infrared spectrophotometry. In: Journal of Vascular Surgery. 2003 ; Vol. 37, No. 5. pp. 1017-1026.
@article{0877d4f90df3420ab1d28d0fc8f3434e,
title = "Localized irregularities in hemoglobin flow and oxygenation in calf muscle in patients with peripheral vascular disease detected with near-infrared spectrophotometry",
abstract = "Purpose: Near-infrared spectrophotometry is used to measure flow, concentration, and oxygenation of hemoglobin in arterioles, capillaries, and venules several centimeters deep in tissue. The purpose of this study was to investigate the distribution of flow, concentration, and oxygenation of hemoglobin in calf muscle in patients with documented peripheral arterial occlusive disease (PVD), patients with risk factors for PVD, and healthy younger subjects at rest. Method: With a frequency-domain near-infrared spectrophotometer and a specially designed probe, we generated maps at 22 locations simultaneously of hemoglobin flow, concentration, and oxygenation, with the venous occlusion method. Eight legs of 7 patients with diagnosed PVD (PVD group), 10 legs of 8 patients with normal ankle-brachial index but with risk factors for PVD (RF group), and 16 legs of 8 healthy subjects (H group) were studied. Results: Global mean values were significantly (P < .05) different between the three groups for oxygen consumption (PVD group, 0.027 ± 0.009 mL/100 g/min; RF group, 0.038 ± 0.017 mL/100 g/min; H group, 0.022 ± 0.020 mL/100 g/min), venous oxygen saturation (PVD, 59.7{\%} ± 15.4{\%}; RF, 69.6{\%} ± 10.5{\%}; H, 80.8{\%} ± 4.5{\%}), and, at 60 s of venous occlusion, concentration changes in oxyhemoglobin (PVD, 4.48 ± 3.25 μmol/L; RF, 8.44 ± 2.33 μmol/L; H, 6.85 ± 4.57 μmol/L), deoxyhemoglobin (PVD, 3.60 ± 0.73 μmol/L; RF, 4.39 ± 1.30 μmol/L; H, 2.36 ± 1.79 μmol/L), and total hemoglobin (PVD, 8.07 ± 3.83 μmol/L; RF, 12.83 ± 2.75 μmol/L; H, 9.21 ± 6.34 μmol/L). No significant difference was found between the three groups for hemoglobin flow (PVD, 0.92 ± 0.69 μmol/100 mL/min; RF, 1.68 ± 0.50 μmol/100 mL/min; H, 1.44 ± 1.17 μmol/100 mL/min) and blood flow (PVD, 0.45 ± 0.28 mL/100 g/min; RF, 0.77 ± 0.21 mL/100 g/min; H, 0.62 ± 0.50 mL/100 g/min). All parameters featured a distribution dependent on location. Conclusion: Mean value for venous oxygen saturation was higher in healthy subjects compared to patients with documented PVD. In patients with PVD, areas of lower oxygenation were clearly discernible. At distal locations of calf muscle, significant correlations between reduced hemoglobin flow, venous oxygen saturation, oxyhemoglobin, and total hemoglobin and reduced ankle-brachial index were found. Maps revealed localized irregularities in oxyhemoglobin, total hemoglobin, and venous oxygen saturation in patients with PVD. Near-infrared spectrophotometry is a noninvasive bedside technique that can enable determination of blood flow and oxygenation in tissue and may provide a method for evaluating patients with PVD.",
author = "Ursula Wolf and Martin Wolf and Choi, {Jee H.} and Moshe Levi and Devasmita Choudhury and Sherri Hull and Daniel Coussirat and Paunescu, {L. Adelina} and Safonova, {Larisa P.} and Antonios Michalos and Mantulin, {William W.} and Enrico Gratton",
year = "2003",
month = "5",
day = "1",
doi = "10.1067/mva.2003.214",
language = "English (US)",
volume = "37",
pages = "1017--1026",
journal = "Journal of Vascular Surgery",
issn = "0741-5214",
publisher = "Mosby Inc.",
number = "5",

}

TY - JOUR

T1 - Localized irregularities in hemoglobin flow and oxygenation in calf muscle in patients with peripheral vascular disease detected with near-infrared spectrophotometry

AU - Wolf, Ursula

AU - Wolf, Martin

AU - Choi, Jee H.

AU - Levi, Moshe

AU - Choudhury, Devasmita

AU - Hull, Sherri

AU - Coussirat, Daniel

AU - Paunescu, L. Adelina

AU - Safonova, Larisa P.

AU - Michalos, Antonios

AU - Mantulin, William W.

AU - Gratton, Enrico

PY - 2003/5/1

Y1 - 2003/5/1

N2 - Purpose: Near-infrared spectrophotometry is used to measure flow, concentration, and oxygenation of hemoglobin in arterioles, capillaries, and venules several centimeters deep in tissue. The purpose of this study was to investigate the distribution of flow, concentration, and oxygenation of hemoglobin in calf muscle in patients with documented peripheral arterial occlusive disease (PVD), patients with risk factors for PVD, and healthy younger subjects at rest. Method: With a frequency-domain near-infrared spectrophotometer and a specially designed probe, we generated maps at 22 locations simultaneously of hemoglobin flow, concentration, and oxygenation, with the venous occlusion method. Eight legs of 7 patients with diagnosed PVD (PVD group), 10 legs of 8 patients with normal ankle-brachial index but with risk factors for PVD (RF group), and 16 legs of 8 healthy subjects (H group) were studied. Results: Global mean values were significantly (P < .05) different between the three groups for oxygen consumption (PVD group, 0.027 ± 0.009 mL/100 g/min; RF group, 0.038 ± 0.017 mL/100 g/min; H group, 0.022 ± 0.020 mL/100 g/min), venous oxygen saturation (PVD, 59.7% ± 15.4%; RF, 69.6% ± 10.5%; H, 80.8% ± 4.5%), and, at 60 s of venous occlusion, concentration changes in oxyhemoglobin (PVD, 4.48 ± 3.25 μmol/L; RF, 8.44 ± 2.33 μmol/L; H, 6.85 ± 4.57 μmol/L), deoxyhemoglobin (PVD, 3.60 ± 0.73 μmol/L; RF, 4.39 ± 1.30 μmol/L; H, 2.36 ± 1.79 μmol/L), and total hemoglobin (PVD, 8.07 ± 3.83 μmol/L; RF, 12.83 ± 2.75 μmol/L; H, 9.21 ± 6.34 μmol/L). No significant difference was found between the three groups for hemoglobin flow (PVD, 0.92 ± 0.69 μmol/100 mL/min; RF, 1.68 ± 0.50 μmol/100 mL/min; H, 1.44 ± 1.17 μmol/100 mL/min) and blood flow (PVD, 0.45 ± 0.28 mL/100 g/min; RF, 0.77 ± 0.21 mL/100 g/min; H, 0.62 ± 0.50 mL/100 g/min). All parameters featured a distribution dependent on location. Conclusion: Mean value for venous oxygen saturation was higher in healthy subjects compared to patients with documented PVD. In patients with PVD, areas of lower oxygenation were clearly discernible. At distal locations of calf muscle, significant correlations between reduced hemoglobin flow, venous oxygen saturation, oxyhemoglobin, and total hemoglobin and reduced ankle-brachial index were found. Maps revealed localized irregularities in oxyhemoglobin, total hemoglobin, and venous oxygen saturation in patients with PVD. Near-infrared spectrophotometry is a noninvasive bedside technique that can enable determination of blood flow and oxygenation in tissue and may provide a method for evaluating patients with PVD.

AB - Purpose: Near-infrared spectrophotometry is used to measure flow, concentration, and oxygenation of hemoglobin in arterioles, capillaries, and venules several centimeters deep in tissue. The purpose of this study was to investigate the distribution of flow, concentration, and oxygenation of hemoglobin in calf muscle in patients with documented peripheral arterial occlusive disease (PVD), patients with risk factors for PVD, and healthy younger subjects at rest. Method: With a frequency-domain near-infrared spectrophotometer and a specially designed probe, we generated maps at 22 locations simultaneously of hemoglobin flow, concentration, and oxygenation, with the venous occlusion method. Eight legs of 7 patients with diagnosed PVD (PVD group), 10 legs of 8 patients with normal ankle-brachial index but with risk factors for PVD (RF group), and 16 legs of 8 healthy subjects (H group) were studied. Results: Global mean values were significantly (P < .05) different between the three groups for oxygen consumption (PVD group, 0.027 ± 0.009 mL/100 g/min; RF group, 0.038 ± 0.017 mL/100 g/min; H group, 0.022 ± 0.020 mL/100 g/min), venous oxygen saturation (PVD, 59.7% ± 15.4%; RF, 69.6% ± 10.5%; H, 80.8% ± 4.5%), and, at 60 s of venous occlusion, concentration changes in oxyhemoglobin (PVD, 4.48 ± 3.25 μmol/L; RF, 8.44 ± 2.33 μmol/L; H, 6.85 ± 4.57 μmol/L), deoxyhemoglobin (PVD, 3.60 ± 0.73 μmol/L; RF, 4.39 ± 1.30 μmol/L; H, 2.36 ± 1.79 μmol/L), and total hemoglobin (PVD, 8.07 ± 3.83 μmol/L; RF, 12.83 ± 2.75 μmol/L; H, 9.21 ± 6.34 μmol/L). No significant difference was found between the three groups for hemoglobin flow (PVD, 0.92 ± 0.69 μmol/100 mL/min; RF, 1.68 ± 0.50 μmol/100 mL/min; H, 1.44 ± 1.17 μmol/100 mL/min) and blood flow (PVD, 0.45 ± 0.28 mL/100 g/min; RF, 0.77 ± 0.21 mL/100 g/min; H, 0.62 ± 0.50 mL/100 g/min). All parameters featured a distribution dependent on location. Conclusion: Mean value for venous oxygen saturation was higher in healthy subjects compared to patients with documented PVD. In patients with PVD, areas of lower oxygenation were clearly discernible. At distal locations of calf muscle, significant correlations between reduced hemoglobin flow, venous oxygen saturation, oxyhemoglobin, and total hemoglobin and reduced ankle-brachial index were found. Maps revealed localized irregularities in oxyhemoglobin, total hemoglobin, and venous oxygen saturation in patients with PVD. Near-infrared spectrophotometry is a noninvasive bedside technique that can enable determination of blood flow and oxygenation in tissue and may provide a method for evaluating patients with PVD.

UR - http://www.scopus.com/inward/record.url?scp=0037696290&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037696290&partnerID=8YFLogxK

U2 - 10.1067/mva.2003.214

DO - 10.1067/mva.2003.214

M3 - Article

C2 - 12756348

AN - SCOPUS:0037696290

VL - 37

SP - 1017

EP - 1026

JO - Journal of Vascular Surgery

JF - Journal of Vascular Surgery

SN - 0741-5214

IS - 5

ER -