Localized recruitment of a chromatin-remodeling activity by an activator in vivo drives transcriptional elongation

Laura L. Corey, Christine S. Weirich, Ivor J. Benjamin, Robert E. Kingston

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

To understand the role of chromatin-remodeling activities in transcription, it is necessary to understand how they interact with transcriptional activators in vivo to regulate the different steps of transcription. Human heat shock factor 1 (HSF1) stimulates both transcriptional initiation and elongation. We replaced mouse HSF1 in fibroblasts with wild-type and mutant human HSF1 constructs and characterized regulation of an endogenous mouse hsp70 gene. A mutation that diminished transcriptional initiation led to twofold reductions in hsp70 mRNA induction and recruitment of a SWI/SNF remodeling complex. In contrast, a mutation that diminished transcriptional elongation abolished induction of full-length mRNA, SWI/SNF recruitment, and chromatin remodeling, but minimally impaired initiation from the hsp70 promoter. Another remodeling factor, SNF2h, is constitutively present at the promoter irrespective of the genotype of HSF1. These data suggest that localized recruitment of SWI/SNF drives a specialized remodeling reaction necessary for the production of full-length hsp70 mRNA.

Original languageEnglish (US)
Pages (from-to)1392-1401
Number of pages10
JournalGenes and Development
Volume17
Issue number11
StatePublished - Jun 1 2003

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Chromatin Assembly and Disassembly
Shock
Hot Temperature
Messenger RNA
Mutation
Fibroblasts
Genotype
Genes

Keywords

  • Chromatin
  • Elongation
  • Heat shock
  • Hsp70
  • SWI/SNF
  • Transcription

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Localized recruitment of a chromatin-remodeling activity by an activator in vivo drives transcriptional elongation. / Corey, Laura L.; Weirich, Christine S.; Benjamin, Ivor J.; Kingston, Robert E.

In: Genes and Development, Vol. 17, No. 11, 01.06.2003, p. 1392-1401.

Research output: Contribution to journalArticle

Corey, Laura L. ; Weirich, Christine S. ; Benjamin, Ivor J. ; Kingston, Robert E. / Localized recruitment of a chromatin-remodeling activity by an activator in vivo drives transcriptional elongation. In: Genes and Development. 2003 ; Vol. 17, No. 11. pp. 1392-1401.
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