Locus ceruleus in rhesus monkey (Macaca mulatta)

A combined histochemical fluorescence, Nissl and silver study

D. C. German, D. M. Bowden

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The locus ceruleus (LC) of the rhesus monkey (Macaca mulatta) was investigated using the histochemical fluorescence method and Nissl and ammoniacal silver strains. Caudally, a few fluorescent cells were observed in the lateral wall of the ventriculus quartus near the velum medullaris superior. Rostrally, the fluorescent cells were compactly clustered and reached their greatest density medial and, to a lesser extent, lateral to the tractus mesencephalicus n. trigemini at the level of the decussation of the nervus trochlearis. In the most rostral plane, fluorescent cells were more diffusely scattered ventral and medial to the pedunculus cerebellaris superior with a few cells situated near the nervus trochlearis. Nissl staining of tissue previously used for histochemical fluorescence showed that fluorescent cells were largely found in a region labelled LC in 2 rhesus monkey brain atlases and erroneously labelled nucleus tractus mesencephalicus n. trigemini in one brain atlas. Ammoniacal silver staining resulted in a dense accumulation of silver granules in the cells found to display a positive reaction for monoamines. The silver stains therefore offer an alternative to the histochemical fluorescence method for identifying the monoamine containing LC neurons.

Original languageEnglish (US)
Pages (from-to)19-29
Number of pages11
JournalJournal of Comparative Neurology
Volume161
Issue number1
StatePublished - 1975

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Locus Coeruleus
Macaca mulatta
Silver
Fluorescence
Atlases
Silver Staining
Brain
Coloring Agents
Staining and Labeling
Neurons

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Locus ceruleus in rhesus monkey (Macaca mulatta) : A combined histochemical fluorescence, Nissl and silver study. / German, D. C.; Bowden, D. M.

In: Journal of Comparative Neurology, Vol. 161, No. 1, 1975, p. 19-29.

Research output: Contribution to journalArticle

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