Long-lasting, experience-dependent alcohol preference in Drosophila

Raniero L. Peru, Shamsideen A. Ojelade, Pranav S. Penninti, Rachel J. Dove, Matthew J. Nye, Summer F. Acevedo, Antonio Lopez, Aylin R. Rodan, Adrian Rothenfluh

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

To understand the molecular and neural mechanisms underlying alcohol addiction, many models ranging from vertebrates to invertebrates have been developed. In Drosophila melanogaster, behavioral paradigms from assaying acute responses to alcohol and to behaviors more closely modeling addiction have emerged in recent years. However, both the CAFÉ assay, similar to a two-bottle choice consumption assay, as well as conditioned odor preference, where ethanol is used as the reinforcer, are labor intensive and have low throughput. To address this limitation, we have established a novel ethanol consumption preference assay, called FRAPPÉ, which allows for fast, high throughput measurement of consumption in individual flies, using a fluorescence plate reader. We show that naïve flies do not prefer to consume ethanol, but various pre-exposures, such as ethanol vapor or voluntary ethanol consumption, induce ethanol preference. This ethanol-primed preference is long lasting and is not driven by calories contained in ethanol during the consumption choice. Our novel experience-dependent model of ethanol preference in Drosophila - a highly genetically tractable organism - therefore recapitulates salient features of human alcohol abuse and will facilitate the molecular understanding of the development of alcohol preference.

Original languageEnglish (US)
Pages (from-to)392-401
Number of pages10
JournalAddiction Biology
Volume19
Issue number3
DOIs
StatePublished - May 2014

Keywords

  • Addiction
  • Drosophila
  • alcohol
  • genetics
  • model organism
  • self-administration

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Pharmacology
  • Psychiatry and Mental health

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  • Cite this

    Peru, R. L., Ojelade, S. A., Penninti, P. S., Dove, R. J., Nye, M. J., Acevedo, S. F., Lopez, A., Rodan, A. R., & Rothenfluh, A. (2014). Long-lasting, experience-dependent alcohol preference in Drosophila. Addiction Biology, 19(3), 392-401. https://doi.org/10.1111/adb.12105