Long-range PCR based sequencing of the highly homologous genes, SFTPA1 and SFTPA2

Christopher J. Horton; Midori Mitui; Nora K. Leos; Christine Kim Garcia; Jason Y. Park

doi:10.1016/j.mcp.2013.01.001

Long-range PCR based sequencing of the highly homologous genes, SFTPA1 and SFTPA2

Christopher J. Horton, Midori Mitui, Nora K. Leos, Christine Kim Garcia, Jason Y. Park

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

In humans, Surfactant Protein A exists as two highly homologous genetic isoforms, SFTPA1 and SFTPA2. Mutations in these two genes are associated with idiopathic pulmonary fibrosis (IPF) and lung cancer. We have developed a Sanger DNA sequencing assay which utilizes long-range PCR to detect mutations in these two genes.

Original language	English (US)
Pages (from-to)	115-117
Number of pages	3
Journal	Molecular and Cellular Probes
Volume	27
Issue number	3-4
DOIs	https://doi.org/10.1016/j.mcp.2013.01.001
State	Published - Jun 2013

Keywords

Allele dropout
DNA sequencing
Long-range PCR
Restriction enzyme
SFTPA1
SFTPA2

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1016/j.mcp.2013.01.001

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title = "Long-range PCR based sequencing of the highly homologous genes, SFTPA1 and SFTPA2",

abstract = "In humans, Surfactant Protein A exists as two highly homologous genetic isoforms, SFTPA1 and SFTPA2. Mutations in these two genes are associated with idiopathic pulmonary fibrosis (IPF) and lung cancer. We have developed a Sanger DNA sequencing assay which utilizes long-range PCR to detect mutations in these two genes.",

keywords = "Allele dropout, DNA sequencing, Long-range PCR, Restriction enzyme, SFTPA1, SFTPA2",

author = "Horton, {Christopher J.} and Midori Mitui and Leos, {Nora K.} and Garcia, {Christine Kim} and Park, {Jason Y.}",

year = "2013",

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doi = "10.1016/j.mcp.2013.01.001",

language = "English (US)",

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journal = "Molecular and Cellular Probes",

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T1 - Long-range PCR based sequencing of the highly homologous genes, SFTPA1 and SFTPA2

AU - Horton, Christopher J.

AU - Mitui, Midori

AU - Leos, Nora K.

AU - Garcia, Christine Kim

AU - Park, Jason Y.

PY - 2013/6

Y1 - 2013/6

N2 - In humans, Surfactant Protein A exists as two highly homologous genetic isoforms, SFTPA1 and SFTPA2. Mutations in these two genes are associated with idiopathic pulmonary fibrosis (IPF) and lung cancer. We have developed a Sanger DNA sequencing assay which utilizes long-range PCR to detect mutations in these two genes.

AB - In humans, Surfactant Protein A exists as two highly homologous genetic isoforms, SFTPA1 and SFTPA2. Mutations in these two genes are associated with idiopathic pulmonary fibrosis (IPF) and lung cancer. We have developed a Sanger DNA sequencing assay which utilizes long-range PCR to detect mutations in these two genes.

KW - Allele dropout

KW - DNA sequencing

KW - Long-range PCR

KW - Restriction enzyme

KW - SFTPA1

KW - SFTPA2

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SP - 115

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JO - Molecular and Cellular Probes

JF - Molecular and Cellular Probes

IS - 3-4

ER -

Long-range PCR based sequencing of the highly homologous genes, SFTPA1 and SFTPA2

Abstract

Keywords

ASJC Scopus subject areas

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