Long-range PCR based sequencing of the highly homologous genes, SFTPA1 and SFTPA2

Christopher J. Horton, Midori Mitui, Nora K. Leos, Christine Kim Garcia, Jason Y. Park

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

In humans, Surfactant Protein A exists as two highly homologous genetic isoforms, SFTPA1 and SFTPA2. Mutations in these two genes are associated with idiopathic pulmonary fibrosis (IPF) and lung cancer. We have developed a Sanger DNA sequencing assay which utilizes long-range PCR to detect mutations in these two genes.

Original languageEnglish (US)
Pages (from-to)115-117
Number of pages3
JournalMolecular and Cellular Probes
Volume27
Issue number3-4
DOIs
Publication statusPublished - Jun 2013

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Keywords

  • Allele dropout
  • DNA sequencing
  • Long-range PCR
  • Restriction enzyme
  • SFTPA1
  • SFTPA2

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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