Long-term culture of fibroblasts in contracted collagen gels: Effects on cell growth and biosynthetic activity

Shigenori Nakagawa, Pamela Pawelek, Frederick Grinnell

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

The purpose of these studies was to analyze the consequences of long-term collagen gel contraction on fibroblast growth and metabolic activity. After 4 weeks, floating gels were 98% contracted, and attached gels were 94% contracted. During this culture period, fibroblasts in floating gels regressed significantly compared to fibroblasts in attached gels, although the cells remaining in the floating gels were viable. In attached gels, fibroblasts were bipolar; whereas in floating gels, fibroblasts were stellate. Therefore, differences between survival of fibroblasts in attached and floating collagen gels might depend on cell shape. Similarly, extracellular matrix organization and its influence on cell shape might control fibroblast proliferation in granulation tissue. During long-term culture of fibroblasts in contracted collagen gels, 70%-80% of the starting collagen was degraded. Collagen synthesized by cells in 4-d cultures was mostly procollagen secreted into the medium. On the other hand, collagen synthesized in 4-week cultures was processed to α(I) chains and incorporated into the matrix. There also were other differences between the proteins synthesized by fibroblasts after short-term and long-term culture in contracted gels. These findings show that fibroblasts in long-term collagen gel cultures express unique growth and biosynthetic characteristics.

Original languageEnglish (US)
Pages (from-to)792-798
Number of pages7
JournalJournal of Investigative Dermatology
Volume93
Issue number6
StatePublished - Dec 1989

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Cell growth
Fibroblasts
Cell culture
Collagen
Gels
Growth
Cell Shape
Procollagen
Granulation
Granulation Tissue
Extracellular Matrix
Tissue

ASJC Scopus subject areas

  • Dermatology

Cite this

Long-term culture of fibroblasts in contracted collagen gels : Effects on cell growth and biosynthetic activity. / Nakagawa, Shigenori; Pawelek, Pamela; Grinnell, Frederick.

In: Journal of Investigative Dermatology, Vol. 93, No. 6, 12.1989, p. 792-798.

Research output: Contribution to journalArticle

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abstract = "The purpose of these studies was to analyze the consequences of long-term collagen gel contraction on fibroblast growth and metabolic activity. After 4 weeks, floating gels were 98{\%} contracted, and attached gels were 94{\%} contracted. During this culture period, fibroblasts in floating gels regressed significantly compared to fibroblasts in attached gels, although the cells remaining in the floating gels were viable. In attached gels, fibroblasts were bipolar; whereas in floating gels, fibroblasts were stellate. Therefore, differences between survival of fibroblasts in attached and floating collagen gels might depend on cell shape. Similarly, extracellular matrix organization and its influence on cell shape might control fibroblast proliferation in granulation tissue. During long-term culture of fibroblasts in contracted collagen gels, 70{\%}-80{\%} of the starting collagen was degraded. Collagen synthesized by cells in 4-d cultures was mostly procollagen secreted into the medium. On the other hand, collagen synthesized in 4-week cultures was processed to α(I) chains and incorporated into the matrix. There also were other differences between the proteins synthesized by fibroblasts after short-term and long-term culture in contracted gels. These findings show that fibroblasts in long-term collagen gel cultures express unique growth and biosynthetic characteristics.",
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