Longitudinal mechanical tension induces growth in the small bowel of juvenile rats

S. D. Safford, A. J. Freemerman, K. M. Safford, R. Bentley, M. A. Skinner

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Introduction: The aim of our study was to apply longitudinal force to the small bowel to increase the length of intestine in juvenile rats. Methods: Fifty juvenile rats had double barrelled, blind loop ostomies created using an isolated segment of bowel. Our intestinal lengthening device was inserted into one of the loops and the second loop served as a control. Once the device was deployed, the experimental, control, and in situ segments of bowel were evaluated for length, weight, histology, and disaccharidase enzyme activity. Results: Mechanical tension increased intestinal length by 149%. The lengthened bowel also exhibited a greater total weight (218%), greater mucosal weight (122%), and increased protein mass (164%) compared with the control limb of bowel. Histologically, there was a markedly increased thickness of the muscularis propria in the lengthened bowel (200% increase compared with the control limb). Functionally, we found increased total disaccharidase activity in the lengthened bowel (between 47% and 350%, depending on the particular enzyme tested; p<0.01). Conclusion: Mechanical tension induces intestinal growth by increasing length, weight of the bowel and mucosa, and protein mass. Histological changes, such as increases in Paneth cells, suggest that increased proliferation and reorganisation of the mucosa and muscularis propria are a response to mechanical tension. Functionally, increased intestinal length corresponds with increased disaccharidase activity, thus implying potential increased absorptive capacity of the lengthened bowel.

Original languageEnglish (US)
Pages (from-to)1085-1090
Number of pages6
JournalGut
Volume54
Issue number8
DOIs
StatePublished - Aug 2005

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Disaccharidases
Weights and Measures
Growth
Mucous Membrane
Extremities
Paneth Cells
Ostomy
Equipment and Supplies
Enzymes
Intestines
Histology
Proteins

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Safford, S. D., Freemerman, A. J., Safford, K. M., Bentley, R., & Skinner, M. A. (2005). Longitudinal mechanical tension induces growth in the small bowel of juvenile rats. Gut, 54(8), 1085-1090. https://doi.org/10.1136/gut.2004.061481

Longitudinal mechanical tension induces growth in the small bowel of juvenile rats. / Safford, S. D.; Freemerman, A. J.; Safford, K. M.; Bentley, R.; Skinner, M. A.

In: Gut, Vol. 54, No. 8, 08.2005, p. 1085-1090.

Research output: Contribution to journalArticle

Safford, SD, Freemerman, AJ, Safford, KM, Bentley, R & Skinner, MA 2005, 'Longitudinal mechanical tension induces growth in the small bowel of juvenile rats', Gut, vol. 54, no. 8, pp. 1085-1090. https://doi.org/10.1136/gut.2004.061481
Safford SD, Freemerman AJ, Safford KM, Bentley R, Skinner MA. Longitudinal mechanical tension induces growth in the small bowel of juvenile rats. Gut. 2005 Aug;54(8):1085-1090. https://doi.org/10.1136/gut.2004.061481
Safford, S. D. ; Freemerman, A. J. ; Safford, K. M. ; Bentley, R. ; Skinner, M. A. / Longitudinal mechanical tension induces growth in the small bowel of juvenile rats. In: Gut. 2005 ; Vol. 54, No. 8. pp. 1085-1090.
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