Looping-out mechanism for resolution of replicative stress at telomeres

Tianpeng Zhang, Zepeng Zhang, Feng Li, Qian Hu, Haiying Liu, Mengfan Tang, Wenbin Ma, Junjiu Huang, Zhou Songyang, Yikang Rong, Shichuan Zhang, Benjamin Pc Chen, Yong Zhao

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Repetitive DNA is prone to replication fork stalling, which can lead to genome instability. Here, we find that replication fork stalling at telomeres leads to the formation of t-circle-tails, a new extrachromosomal structure that consists of circular telomeric DNA with a single-stranded tail. Structurally, the t-circle-tail resembles cyclized leading or lagging replication intermediates that are excised from the genome by topoisomerase II-mediated cleavage. We also show that the DNA damage repair machinery NHEJ is required for the formation of t-circle-tails and for the resolution of stalled replication forks, suggesting that NHEJ, which is normally constitutively suppressed at telomeres, is activated in the context of replication stress. Inhibition of NHEJ or knockout of DNA-PKcs impairs telomere replication, leading to multiple-telomere sites (MTS) and telomere shortening. Collectively, our results support a "looping-out" mechanism, in which the stalled replication fork is cut out and cyclized to form t-circle-tails, and broken DNA is religated. The telomere loss induced by replication stress may serve as a new factor that drives replicative senescence and cell aging.

Original languageEnglish (US)
JournalEMBO Reports
DOIs
StateAccepted/In press - 2017

Fingerprint

Telomere
DNA
Cell Aging
Genes
Telomere Shortening
Circular DNA
Type II DNA Topoisomerase
Genomic Instability
DNA Repair
DNA Damage
Machinery
Repair
Aging of materials
Genome

Keywords

  • NHEJ
  • Looping-out
  • Replication fork stalling
  • Telomeres
  • Topoisomerase II

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Zhang, T., Zhang, Z., Li, F., Hu, Q., Liu, H., Tang, M., ... Zhao, Y. (Accepted/In press). Looping-out mechanism for resolution of replicative stress at telomeres. EMBO Reports. https://doi.org/10.15252/embr.201643866

Looping-out mechanism for resolution of replicative stress at telomeres. / Zhang, Tianpeng; Zhang, Zepeng; Li, Feng; Hu, Qian; Liu, Haiying; Tang, Mengfan; Ma, Wenbin; Huang, Junjiu; Songyang, Zhou; Rong, Yikang; Zhang, Shichuan; Chen, Benjamin Pc; Zhao, Yong.

In: EMBO Reports, 2017.

Research output: Contribution to journalArticle

Zhang, T, Zhang, Z, Li, F, Hu, Q, Liu, H, Tang, M, Ma, W, Huang, J, Songyang, Z, Rong, Y, Zhang, S, Chen, BP & Zhao, Y 2017, 'Looping-out mechanism for resolution of replicative stress at telomeres', EMBO Reports. https://doi.org/10.15252/embr.201643866
Zhang, Tianpeng ; Zhang, Zepeng ; Li, Feng ; Hu, Qian ; Liu, Haiying ; Tang, Mengfan ; Ma, Wenbin ; Huang, Junjiu ; Songyang, Zhou ; Rong, Yikang ; Zhang, Shichuan ; Chen, Benjamin Pc ; Zhao, Yong. / Looping-out mechanism for resolution of replicative stress at telomeres. In: EMBO Reports. 2017.
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