Loss of diacylglycerol kinase epsilon in mice causes endothelial distress and impairs glomerular Cox-2 and PGE2 production

Jili Zhu, Moumita Chaki, Dongmei Lu, Chongyu Ren, Shan Shan Wang, Alysha Rauhauser, Binghua Li, Susan Zimmerman, Bokkyoo Jun, Yong Du, Komal Vadnagara, Hanquin Wang, Sarah Elhadi, Richard J. Quigg, Matthew K. Topham, Chandra Mohan, Fatih Ozaltin, Xin J. Zhou, Denise K. Marciano, Nicolas G. BazanMassimo Attanasio

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Thrombotic microangiopathy (TMA) is a disorder characterized by microvascular occlusion that can lead to thrombocytopenia, hemolytic anemia, and glomerular damage. Complement activation is the central event in most cases of TMA. Primary forms of TMA are caused by mutations in genes encoding components of the complement or regulators of the complement cascade. Recently, we and others have described a genetic form of TMA caused by mutations in the gene diacylglycerol kinase-ε (DGKE) that encodes the lipid kinase DGKε (Lemaire M, Fremeaux-Bacchi V, Schaefer F, Choi MR, Tang WH, Le Quintrec M, Fakhouri F, Taque S, Nobili F, Martinez F, Ji WZ, Overton JD, Mane SM, Nurnberg G, Altmuller J, Thiele H, Morin D, Deschenes G, Baudouin V, Llanas B, Collard L, Majid MA, Simkova E, Nurnberg P, Rioux-Leclerc N, Moeckel GW, Gubler MC, Hwa J, Loirat C, Lifton RP. Nat Genet 45: 531–536, 2013; Ozaltin F, Li BH, Rauhauser A, An SW, Soylemezoglu O, Gonul II, Taskiran EZ, Ibsirlioglu T, Korkmaz E, Bilginer Y, Duzova A, Ozen S, Topaloglu R, Besbas N, Ashraf S, Du Y, Liang CY, Chen P, Lu DM, Vadnagara K, Arbuckle S, Lewis D, Wakeland B, Quigg RJ, Ransom RF, Wakeland EK, Topham MK, Bazan NG, Mohan C, Hildebrandt F, Bakkaloglu A, Huang CL, Attanasio M. J Am Soc Nephrol 24: 377–384, 2013). DGKε is unrelated to the complement pathway, which suggests that unidentified pathogenic mechanisms independent of complement dysregulation may result in TMA. Studying Dgke knockout mice may help to understand the pathogenesis of this disease, but no glomerular phenotype has been described in these animals so far. Here we report that Dgke null mice present subclinical microscopic anomalies of the glomerular endothelium and basal membrane that worsen with age and develop glomerular capillary occlusion when exposed to nephrotoxic serum. We found that induction of cyclooxygenase-2 and of the proangiogenic prostaglandin E2 are impaired in Dgke null kidneys and are associated with reduced expression of the antithrombotic cell adhesion molecule platelet endothelial cell adhesion molecule-1/CD31 in the glomerular endothelium. Notably, prostaglandin E2 supplementation was able to rescue motility defects of Dgke knockdown cells in vitro and to restore angiogenesis in a test in vivo. Our results unveil an unexpected role of Dgke in the induction of cyclooxygenase-2 and in the regulation of glomerular prostanoids synthesis under stress.

Original languageEnglish (US)
Pages (from-to)F895-F908
JournalAmerican Journal of Physiology - Renal Physiology
Volume310
Issue number9
DOIs
StatePublished - May 1 2016

Keywords

  • DGKE
  • Hemolytic uremic syndrome
  • Thrombotic microangiopathy

ASJC Scopus subject areas

  • Physiology
  • Urology

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    Zhu, J., Chaki, M., Lu, D., Ren, C., Wang, S. S., Rauhauser, A., Li, B., Zimmerman, S., Jun, B., Du, Y., Vadnagara, K., Wang, H., Elhadi, S., Quigg, R. J., Topham, M. K., Mohan, C., Ozaltin, F., Zhou, X. J., Marciano, D. K., ... Attanasio, M. (2016). Loss of diacylglycerol kinase epsilon in mice causes endothelial distress and impairs glomerular Cox-2 and PGE2 production. American Journal of Physiology - Renal Physiology, 310(9), F895-F908. https://doi.org/10.1152/ajprenal.00431.2015