Loss of function of endothelin-2 leads to reduced ovulation and CL formation

Joseph A. Cacioppo, Sang Wook Oh, Hey Young Kim, Jongki Cho, Po Ching Patrick Lin, Masashi Yanagisawa, CheMyong Ko

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Endothelin-2 (EDN2), a potent vasoconstrictive peptide, is transiently produced by periovulatory follicles at the time of ovulation when corpus luteum (CL) formation begins. EDN2 induces contraction of ovarian smooth muscles ex vivo via an endothelin receptor A-mediated pathway. In this study, we aimed to determine if EDN2 is required for normal ovulation and subsequent CL formation in vivo. In the ovaries of a mouse model that globally lacks the Edn2 gene (Edn2 knockout mouse; Edn2KO), histology showed that post-pubertal Edn2KO mice possess follicles of all developmental stages, but no corpora lutea. When exogenous gonadotropins were injected to induce super-ovulation, Edn2KO mice exhibited significantly impaired ovulation and CL formation compared to control littermates. Edn2KO ovaries that did ovulate in response to gonadotropins did not contain histologically and functionally identifiable CL. Intra-ovarian injection of EDN2 peptide results suggest partial induction of ovulation in Edn2KO mice. Endothelin receptor antagonism in wild type mice similarly disrupted ovulation, CL formation, and progesterone secretion. Overall, this study suggests that EDN2 is necessary for normal ovulation and CL formation.

Original languageEnglish (US)
Article numbere96115
JournalPLoS One
Volume9
Issue number4
DOIs
StatePublished - Apr 24 2014

Fingerprint

Endothelin-2
endothelins
Corpus Luteum
Ovulation
corpus luteum
ovulation
Gonadotropins
mice
gonadotropins
Endothelin A Receptors
Endothelin Receptors
Peptides
Ovary
Histology
peptides
Progesterone
Gene Knockout Techniques
Muscle
receptors
superovulation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Cacioppo, J. A., Oh, S. W., Kim, H. Y., Cho, J., Lin, P. C. P., Yanagisawa, M., & Ko, C. (2014). Loss of function of endothelin-2 leads to reduced ovulation and CL formation. PLoS One, 9(4), [e96115]. https://doi.org/10.1371/journal.pone.0096115

Loss of function of endothelin-2 leads to reduced ovulation and CL formation. / Cacioppo, Joseph A.; Oh, Sang Wook; Kim, Hey Young; Cho, Jongki; Lin, Po Ching Patrick; Yanagisawa, Masashi; Ko, CheMyong.

In: PLoS One, Vol. 9, No. 4, e96115, 24.04.2014.

Research output: Contribution to journalArticle

Cacioppo, Joseph A. ; Oh, Sang Wook ; Kim, Hey Young ; Cho, Jongki ; Lin, Po Ching Patrick ; Yanagisawa, Masashi ; Ko, CheMyong. / Loss of function of endothelin-2 leads to reduced ovulation and CL formation. In: PLoS One. 2014 ; Vol. 9, No. 4.
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