Loss of function of the Prx1 and Prx2 hameobox genes alters architecture of the great elastic arteries and ductus arteriosus

Maarten Bergwerff, Adriana C. Gittenberger-de Groot, Lambertus J. Wisse, Marco C. DeRuiter, Andy Wessels, James F. Martin, Eric N. Olson, Michael J. Kern

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Prx1 (MHox) and Prx2 (S8) are non-clustered homeobox genes that are expressed in a complex, mostly mesenchyme-specific pattern throughout embryogenesis. The expression pattern and gene-targeted mice previously revealed a major role for Prx1 in skeletogenesis. In addition, specific and high expression of both Prx genes was reported in the developing cardiovascular system, predominantly in prospective connective tissues of the heart and in the great arteries and veins. We examined embryos of previously generated gene-targeted mice. Prx2-/- mutants were viable and did not show cardiovascular malformations. Intracardiac morphology of Prx1-/- and Prx1/Prx2-combined null mutants also appeared normal throughout development. However, the Prx1-/- and Prx1/Prx2 double-null mutants showed a vascular abnormality with an abnormal positioning and awkward curvature of the aortic arch in addition to a misdirected and elongated ductus arteriosus, and in two of seven combined mutants, an anomalous retro-oesophageal right subclavian artery. Generally, all great arteries appeared to run somewhat tortuously through the surrounding mesenchyme. The vascular histology and vessel wall thickness were normal in all mutants. Prx1-/- and Prx double-gene-targeted mice revealed similar spectra of vascular anomalies, but double mutants appeared to be more seriously affected. The current findings suggest that other genes may compensate for the loss of Prx in the heart, but, in contrast, our data support a role for Prx in the development of vascular and perivascular matrix.

Original languageEnglish (US)
Pages (from-to)12-19
Number of pages8
JournalVirchows Archiv
Volume436
Issue number1
StatePublished - 2000

Fingerprint

Ductus Arteriosus
Blood Vessels
Arteries
Mesoderm
Genes
Subclavian Artery
Homeobox Genes
Cardiovascular System
Thoracic Aorta
Connective Tissue
Embryonic Development
Veins
Histology
Embryonic Structures
Gene Expression

Keywords

  • Heart development
  • Paired-related homeobox
  • Pharyngeal arch arteries
  • Vascular matrix

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Bergwerff, M., Gittenberger-de Groot, A. C., Wisse, L. J., DeRuiter, M. C., Wessels, A., Martin, J. F., ... Kern, M. J. (2000). Loss of function of the Prx1 and Prx2 hameobox genes alters architecture of the great elastic arteries and ductus arteriosus. Virchows Archiv, 436(1), 12-19.

Loss of function of the Prx1 and Prx2 hameobox genes alters architecture of the great elastic arteries and ductus arteriosus. / Bergwerff, Maarten; Gittenberger-de Groot, Adriana C.; Wisse, Lambertus J.; DeRuiter, Marco C.; Wessels, Andy; Martin, James F.; Olson, Eric N.; Kern, Michael J.

In: Virchows Archiv, Vol. 436, No. 1, 2000, p. 12-19.

Research output: Contribution to journalArticle

Bergwerff, M, Gittenberger-de Groot, AC, Wisse, LJ, DeRuiter, MC, Wessels, A, Martin, JF, Olson, EN & Kern, MJ 2000, 'Loss of function of the Prx1 and Prx2 hameobox genes alters architecture of the great elastic arteries and ductus arteriosus', Virchows Archiv, vol. 436, no. 1, pp. 12-19.
Bergwerff M, Gittenberger-de Groot AC, Wisse LJ, DeRuiter MC, Wessels A, Martin JF et al. Loss of function of the Prx1 and Prx2 hameobox genes alters architecture of the great elastic arteries and ductus arteriosus. Virchows Archiv. 2000;436(1):12-19.
Bergwerff, Maarten ; Gittenberger-de Groot, Adriana C. ; Wisse, Lambertus J. ; DeRuiter, Marco C. ; Wessels, Andy ; Martin, James F. ; Olson, Eric N. ; Kern, Michael J. / Loss of function of the Prx1 and Prx2 hameobox genes alters architecture of the great elastic arteries and ductus arteriosus. In: Virchows Archiv. 2000 ; Vol. 436, No. 1. pp. 12-19.
@article{198d5d7585ab42e7af6890475ddbf5b1,
title = "Loss of function of the Prx1 and Prx2 hameobox genes alters architecture of the great elastic arteries and ductus arteriosus",
abstract = "Prx1 (MHox) and Prx2 (S8) are non-clustered homeobox genes that are expressed in a complex, mostly mesenchyme-specific pattern throughout embryogenesis. The expression pattern and gene-targeted mice previously revealed a major role for Prx1 in skeletogenesis. In addition, specific and high expression of both Prx genes was reported in the developing cardiovascular system, predominantly in prospective connective tissues of the heart and in the great arteries and veins. We examined embryos of previously generated gene-targeted mice. Prx2-/- mutants were viable and did not show cardiovascular malformations. Intracardiac morphology of Prx1-/- and Prx1/Prx2-combined null mutants also appeared normal throughout development. However, the Prx1-/- and Prx1/Prx2 double-null mutants showed a vascular abnormality with an abnormal positioning and awkward curvature of the aortic arch in addition to a misdirected and elongated ductus arteriosus, and in two of seven combined mutants, an anomalous retro-oesophageal right subclavian artery. Generally, all great arteries appeared to run somewhat tortuously through the surrounding mesenchyme. The vascular histology and vessel wall thickness were normal in all mutants. Prx1-/- and Prx double-gene-targeted mice revealed similar spectra of vascular anomalies, but double mutants appeared to be more seriously affected. The current findings suggest that other genes may compensate for the loss of Prx in the heart, but, in contrast, our data support a role for Prx in the development of vascular and perivascular matrix.",
keywords = "Heart development, Paired-related homeobox, Pharyngeal arch arteries, Vascular matrix",
author = "Maarten Bergwerff and {Gittenberger-de Groot}, {Adriana C.} and Wisse, {Lambertus J.} and DeRuiter, {Marco C.} and Andy Wessels and Martin, {James F.} and Olson, {Eric N.} and Kern, {Michael J.}",
year = "2000",
language = "English (US)",
volume = "436",
pages = "12--19",
journal = "Virchows Archiv",
issn = "0945-6317",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Loss of function of the Prx1 and Prx2 hameobox genes alters architecture of the great elastic arteries and ductus arteriosus

AU - Bergwerff, Maarten

AU - Gittenberger-de Groot, Adriana C.

AU - Wisse, Lambertus J.

AU - DeRuiter, Marco C.

AU - Wessels, Andy

AU - Martin, James F.

AU - Olson, Eric N.

AU - Kern, Michael J.

PY - 2000

Y1 - 2000

N2 - Prx1 (MHox) and Prx2 (S8) are non-clustered homeobox genes that are expressed in a complex, mostly mesenchyme-specific pattern throughout embryogenesis. The expression pattern and gene-targeted mice previously revealed a major role for Prx1 in skeletogenesis. In addition, specific and high expression of both Prx genes was reported in the developing cardiovascular system, predominantly in prospective connective tissues of the heart and in the great arteries and veins. We examined embryos of previously generated gene-targeted mice. Prx2-/- mutants were viable and did not show cardiovascular malformations. Intracardiac morphology of Prx1-/- and Prx1/Prx2-combined null mutants also appeared normal throughout development. However, the Prx1-/- and Prx1/Prx2 double-null mutants showed a vascular abnormality with an abnormal positioning and awkward curvature of the aortic arch in addition to a misdirected and elongated ductus arteriosus, and in two of seven combined mutants, an anomalous retro-oesophageal right subclavian artery. Generally, all great arteries appeared to run somewhat tortuously through the surrounding mesenchyme. The vascular histology and vessel wall thickness were normal in all mutants. Prx1-/- and Prx double-gene-targeted mice revealed similar spectra of vascular anomalies, but double mutants appeared to be more seriously affected. The current findings suggest that other genes may compensate for the loss of Prx in the heart, but, in contrast, our data support a role for Prx in the development of vascular and perivascular matrix.

AB - Prx1 (MHox) and Prx2 (S8) are non-clustered homeobox genes that are expressed in a complex, mostly mesenchyme-specific pattern throughout embryogenesis. The expression pattern and gene-targeted mice previously revealed a major role for Prx1 in skeletogenesis. In addition, specific and high expression of both Prx genes was reported in the developing cardiovascular system, predominantly in prospective connective tissues of the heart and in the great arteries and veins. We examined embryos of previously generated gene-targeted mice. Prx2-/- mutants were viable and did not show cardiovascular malformations. Intracardiac morphology of Prx1-/- and Prx1/Prx2-combined null mutants also appeared normal throughout development. However, the Prx1-/- and Prx1/Prx2 double-null mutants showed a vascular abnormality with an abnormal positioning and awkward curvature of the aortic arch in addition to a misdirected and elongated ductus arteriosus, and in two of seven combined mutants, an anomalous retro-oesophageal right subclavian artery. Generally, all great arteries appeared to run somewhat tortuously through the surrounding mesenchyme. The vascular histology and vessel wall thickness were normal in all mutants. Prx1-/- and Prx double-gene-targeted mice revealed similar spectra of vascular anomalies, but double mutants appeared to be more seriously affected. The current findings suggest that other genes may compensate for the loss of Prx in the heart, but, in contrast, our data support a role for Prx in the development of vascular and perivascular matrix.

KW - Heart development

KW - Paired-related homeobox

KW - Pharyngeal arch arteries

KW - Vascular matrix

UR - http://www.scopus.com/inward/record.url?scp=0034004138&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034004138&partnerID=8YFLogxK

M3 - Article

C2 - 10664157

AN - SCOPUS:0034004138

VL - 436

SP - 12

EP - 19

JO - Virchows Archiv

JF - Virchows Archiv

SN - 0945-6317

IS - 1

ER -