Loss of Goosecoid-like and DiGeorge syndrome critical region 14 in interpeduncular nucleus results in altered regulation of rapid eye movement sleep

Hiromasa Funato, Makito Sato, Christopher M. Sinton, Laurent Gautron, S. Clay Williams, Amber Skach, Joel K. Elmquist, Arthur I. Skoultchi, Masashi Yanagisawa

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Sleep and wakefulness are regulated primarily by inhibitory interactions between the hypothalamus and brainstem. The expression of the states of rapid eye movement (REM) sleep and non-REM(NREM) sleep also are correlated with the activity of groups of REM-off and REM-on neurons in the dorsal brainstem. However, the contribution of ventral brainstem nuclei to sleep regulation has been little characterized to date. Here we examined sleep and wakefulness in mice deficient in a homeobox transcription factor, Goosecoid-like (Gscl), which is one of the genes deleted in DiGeorge syndrome or 22q11 deletion syndrome. The expression of Gscl is restricted to the interpeduncular nucleus (IP) in the ventral region of the midbrain-hind-brain transition. The IP has reciprocal connections with several cell groups implicated in sleep/wakefulness regulation. Although Gscl-/-mice have apparently normal anatomy and connections of the IP, they exhibited a reduced total time spent in REM sleep and fewer REMsleep episodes. In addition, Gscl-/- mice showed reduced theta power during REM sleep and increased arousability during REM sleep. Gscl-/- mice also lacked the expression of DiGeorge syndrome critical region 14 (Dgcr14) in the IP. These results indicate that the absence of Gscl and Dgcr14 in the IP results in altered regulation of REM sleep.

Original languageEnglish (US)
Pages (from-to)18155-18160
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number42
DOIs
StatePublished - Oct 19 2010

Fingerprint

DiGeorge Syndrome
REM Sleep
Sleep
Wakefulness
Brain Stem
Goosecoid Protein
22q11 Deletion Syndrome
Interpeduncular Nucleus
Rhombencephalon
Homeobox Genes
Eye Movements
Mesencephalon
Hypothalamus
Anatomy

Keywords

  • Homeobox transcription factor
  • Mouse behavior
  • Ventral brainstem

ASJC Scopus subject areas

  • General

Cite this

Loss of Goosecoid-like and DiGeorge syndrome critical region 14 in interpeduncular nucleus results in altered regulation of rapid eye movement sleep. / Funato, Hiromasa; Sato, Makito; Sinton, Christopher M.; Gautron, Laurent; Williams, S. Clay; Skach, Amber; Elmquist, Joel K.; Skoultchi, Arthur I.; Yanagisawa, Masashi.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 42, 19.10.2010, p. 18155-18160.

Research output: Contribution to journalArticle

@article{f8bbaf4a86d440fdaad1f3930387c71b,
title = "Loss of Goosecoid-like and DiGeorge syndrome critical region 14 in interpeduncular nucleus results in altered regulation of rapid eye movement sleep",
abstract = "Sleep and wakefulness are regulated primarily by inhibitory interactions between the hypothalamus and brainstem. The expression of the states of rapid eye movement (REM) sleep and non-REM(NREM) sleep also are correlated with the activity of groups of REM-off and REM-on neurons in the dorsal brainstem. However, the contribution of ventral brainstem nuclei to sleep regulation has been little characterized to date. Here we examined sleep and wakefulness in mice deficient in a homeobox transcription factor, Goosecoid-like (Gscl), which is one of the genes deleted in DiGeorge syndrome or 22q11 deletion syndrome. The expression of Gscl is restricted to the interpeduncular nucleus (IP) in the ventral region of the midbrain-hind-brain transition. The IP has reciprocal connections with several cell groups implicated in sleep/wakefulness regulation. Although Gscl-/-mice have apparently normal anatomy and connections of the IP, they exhibited a reduced total time spent in REM sleep and fewer REMsleep episodes. In addition, Gscl-/- mice showed reduced theta power during REM sleep and increased arousability during REM sleep. Gscl-/- mice also lacked the expression of DiGeorge syndrome critical region 14 (Dgcr14) in the IP. These results indicate that the absence of Gscl and Dgcr14 in the IP results in altered regulation of REM sleep.",
keywords = "Homeobox transcription factor, Mouse behavior, Ventral brainstem",
author = "Hiromasa Funato and Makito Sato and Sinton, {Christopher M.} and Laurent Gautron and Williams, {S. Clay} and Amber Skach and Elmquist, {Joel K.} and Skoultchi, {Arthur I.} and Masashi Yanagisawa",
year = "2010",
month = "10",
day = "19",
doi = "10.1073/pnas.1012764107",
language = "English (US)",
volume = "107",
pages = "18155--18160",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "42",

}

TY - JOUR

T1 - Loss of Goosecoid-like and DiGeorge syndrome critical region 14 in interpeduncular nucleus results in altered regulation of rapid eye movement sleep

AU - Funato, Hiromasa

AU - Sato, Makito

AU - Sinton, Christopher M.

AU - Gautron, Laurent

AU - Williams, S. Clay

AU - Skach, Amber

AU - Elmquist, Joel K.

AU - Skoultchi, Arthur I.

AU - Yanagisawa, Masashi

PY - 2010/10/19

Y1 - 2010/10/19

N2 - Sleep and wakefulness are regulated primarily by inhibitory interactions between the hypothalamus and brainstem. The expression of the states of rapid eye movement (REM) sleep and non-REM(NREM) sleep also are correlated with the activity of groups of REM-off and REM-on neurons in the dorsal brainstem. However, the contribution of ventral brainstem nuclei to sleep regulation has been little characterized to date. Here we examined sleep and wakefulness in mice deficient in a homeobox transcription factor, Goosecoid-like (Gscl), which is one of the genes deleted in DiGeorge syndrome or 22q11 deletion syndrome. The expression of Gscl is restricted to the interpeduncular nucleus (IP) in the ventral region of the midbrain-hind-brain transition. The IP has reciprocal connections with several cell groups implicated in sleep/wakefulness regulation. Although Gscl-/-mice have apparently normal anatomy and connections of the IP, they exhibited a reduced total time spent in REM sleep and fewer REMsleep episodes. In addition, Gscl-/- mice showed reduced theta power during REM sleep and increased arousability during REM sleep. Gscl-/- mice also lacked the expression of DiGeorge syndrome critical region 14 (Dgcr14) in the IP. These results indicate that the absence of Gscl and Dgcr14 in the IP results in altered regulation of REM sleep.

AB - Sleep and wakefulness are regulated primarily by inhibitory interactions between the hypothalamus and brainstem. The expression of the states of rapid eye movement (REM) sleep and non-REM(NREM) sleep also are correlated with the activity of groups of REM-off and REM-on neurons in the dorsal brainstem. However, the contribution of ventral brainstem nuclei to sleep regulation has been little characterized to date. Here we examined sleep and wakefulness in mice deficient in a homeobox transcription factor, Goosecoid-like (Gscl), which is one of the genes deleted in DiGeorge syndrome or 22q11 deletion syndrome. The expression of Gscl is restricted to the interpeduncular nucleus (IP) in the ventral region of the midbrain-hind-brain transition. The IP has reciprocal connections with several cell groups implicated in sleep/wakefulness regulation. Although Gscl-/-mice have apparently normal anatomy and connections of the IP, they exhibited a reduced total time spent in REM sleep and fewer REMsleep episodes. In addition, Gscl-/- mice showed reduced theta power during REM sleep and increased arousability during REM sleep. Gscl-/- mice also lacked the expression of DiGeorge syndrome critical region 14 (Dgcr14) in the IP. These results indicate that the absence of Gscl and Dgcr14 in the IP results in altered regulation of REM sleep.

KW - Homeobox transcription factor

KW - Mouse behavior

KW - Ventral brainstem

UR - http://www.scopus.com/inward/record.url?scp=78149240061&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78149240061&partnerID=8YFLogxK

U2 - 10.1073/pnas.1012764107

DO - 10.1073/pnas.1012764107

M3 - Article

C2 - 20921407

AN - SCOPUS:78149240061

VL - 107

SP - 18155

EP - 18160

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 42

ER -