Loss of MAX results in meiotic entry in mouse embryonic and germline stem cells

Ayumu Suzuki, Masataka Hirasaki, Tomoaki Hishida, Jun Wu, Daiji Okamura, Atsushi Ueda, Masazumi Nishimoto, Yutaka Nakachi, Yosuke Mizuno, Yasushi Okazaki, Yasuhisa Matsui, Juan Carlos Izpisua Belmonte, Akihiko Okuda

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Meiosis is a unique process that allows the generation of reproductive cells. It remains largely unknown how meiosis is initiated in germ cells and why non-germline cells do not undergo meiosis. We previously demonstrated that knockdown of Max expression, a gene encoding a partner of MYC family proteins, strongly activates expression of germ cell-related genes in ESCs. Here we find that complete ablation of Max expression in ESCs results in profound cytological changes reminiscent of cells undergoing meiotic cell division. Furthermore, our analyses uncovers that Max expression is transiently attenuated in germ cells undergoing meiosis in vivo and its forced reduction induces meiosis-like cytological changes in cultured germline stem cells. Mechanistically, Max depletion alterations are, in part, due to impairment of the function of an atypical PRC1 complex (PRC1.6), in which MAX is one of the components. Our data highlight MAX as a new regulator of meiotic onset.

Original languageEnglish (US)
Article number11056
JournalNature communications
Volume7
DOIs
StatePublished - Mar 30 2016
Externally publishedYes

Fingerprint

stem cells
Meiosis
Stem cells
entry
mice
Cells
Germ Cells
genes
cells
cell division
Gene encoding
regulators
impairment
Ablation
cultured cells
ablation
coding
depletion
Genes
Cell Division

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Suzuki, A., Hirasaki, M., Hishida, T., Wu, J., Okamura, D., Ueda, A., ... Okuda, A. (2016). Loss of MAX results in meiotic entry in mouse embryonic and germline stem cells. Nature communications, 7, [11056]. https://doi.org/10.1038/ncomms11056

Loss of MAX results in meiotic entry in mouse embryonic and germline stem cells. / Suzuki, Ayumu; Hirasaki, Masataka; Hishida, Tomoaki; Wu, Jun; Okamura, Daiji; Ueda, Atsushi; Nishimoto, Masazumi; Nakachi, Yutaka; Mizuno, Yosuke; Okazaki, Yasushi; Matsui, Yasuhisa; Belmonte, Juan Carlos Izpisua; Okuda, Akihiko.

In: Nature communications, Vol. 7, 11056, 30.03.2016.

Research output: Contribution to journalArticle

Suzuki, A, Hirasaki, M, Hishida, T, Wu, J, Okamura, D, Ueda, A, Nishimoto, M, Nakachi, Y, Mizuno, Y, Okazaki, Y, Matsui, Y, Belmonte, JCI & Okuda, A 2016, 'Loss of MAX results in meiotic entry in mouse embryonic and germline stem cells', Nature communications, vol. 7, 11056. https://doi.org/10.1038/ncomms11056
Suzuki, Ayumu ; Hirasaki, Masataka ; Hishida, Tomoaki ; Wu, Jun ; Okamura, Daiji ; Ueda, Atsushi ; Nishimoto, Masazumi ; Nakachi, Yutaka ; Mizuno, Yosuke ; Okazaki, Yasushi ; Matsui, Yasuhisa ; Belmonte, Juan Carlos Izpisua ; Okuda, Akihiko. / Loss of MAX results in meiotic entry in mouse embryonic and germline stem cells. In: Nature communications. 2016 ; Vol. 7.
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