Loss of stromal Caveolin-1 expression in malignant melanoma metastases predicts poor survival

Karen N. Wu, Maria Queenan, Jonathan R. Brody, Magdalena Potoczek, Federica Sotgia, Michael P. Lisanti, Agnieszka K. Witkiewicz

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Caveolins are the principal protein component of caveolae, plasma membrane invaginations found in most cell types. Caveolin-1 (Cav-1) plays a major role in oncogenesis through its various functions in lipid transport, membrane trafficking and signal transduction. Increased expression of Cav-1 in tumor cells has been associated with aggressiveness and poor survival. More recently, loss of stromal Cav-1 expression was linked to poor survival and increased metastatic potential in breast and prostate cancer. To date, there is no study addressing the clinical significance of Cav-1 expression in malignant melanoma (MM). Our study consisted of 44 cases of MM: 12 MM lymph node metastases from patients with short survival, 12 MM lymph node metastases from patients with long survival and 20 primary MM. All cases were stained with Cav?1 antibodies. Cav-1 expression in melanoma and stromal cells was quantified using a 3 point scale: 0 = no staining, 1 = diffuse weak staining or strong staining in <30% of cells and 2 = diffuse strong staining. A score of 0-1 represented low Cav-1 expression and a score of 2 represented high Cav-1 expression. In patients with MM lymph node metastases, a low stromal Cav-1 expression was associated with shorter survival when compared with the high stromal Cav-1 expression group (median survival 252 days vs. 3,508 days, p-value 0.005). Conversely, high Cav-1 expression in melanoma cells was associated with a longer survival in primary MM (p < 0.0001). In conclusion, high expression of stromal Cav-1 correlates with longer survival in malignant melanoma metastases and high expression of Cav-1 in melanoma cells correlates with longer survival in primary malignant melanoma.

Original languageEnglish (US)
Pages (from-to)4250-4255
Number of pages6
JournalCell Cycle
Volume10
Issue number24
DOIs
StatePublished - Dec 15 2011

Fingerprint

Caveolin 1
Melanoma
Neoplasm Metastasis
Survival
Staining and Labeling
Lymph Nodes
Caveolins
Caveolae
Membrane Lipids
Stromal Cells
Signal Transduction
Prostatic Neoplasms
Carcinogenesis

Keywords

  • Caveolin-1
  • Melanoma
  • Metastases
  • Stroma
  • Survival

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Developmental Biology

Cite this

Wu, K. N., Queenan, M., Brody, J. R., Potoczek, M., Sotgia, F., Lisanti, M. P., & Witkiewicz, A. K. (2011). Loss of stromal Caveolin-1 expression in malignant melanoma metastases predicts poor survival. Cell Cycle, 10(24), 4250-4255. https://doi.org/10.4161/cc.10.24.18551

Loss of stromal Caveolin-1 expression in malignant melanoma metastases predicts poor survival. / Wu, Karen N.; Queenan, Maria; Brody, Jonathan R.; Potoczek, Magdalena; Sotgia, Federica; Lisanti, Michael P.; Witkiewicz, Agnieszka K.

In: Cell Cycle, Vol. 10, No. 24, 15.12.2011, p. 4250-4255.

Research output: Contribution to journalArticle

Wu, KN, Queenan, M, Brody, JR, Potoczek, M, Sotgia, F, Lisanti, MP & Witkiewicz, AK 2011, 'Loss of stromal Caveolin-1 expression in malignant melanoma metastases predicts poor survival', Cell Cycle, vol. 10, no. 24, pp. 4250-4255. https://doi.org/10.4161/cc.10.24.18551
Wu KN, Queenan M, Brody JR, Potoczek M, Sotgia F, Lisanti MP et al. Loss of stromal Caveolin-1 expression in malignant melanoma metastases predicts poor survival. Cell Cycle. 2011 Dec 15;10(24):4250-4255. https://doi.org/10.4161/cc.10.24.18551
Wu, Karen N. ; Queenan, Maria ; Brody, Jonathan R. ; Potoczek, Magdalena ; Sotgia, Federica ; Lisanti, Michael P. ; Witkiewicz, Agnieszka K. / Loss of stromal Caveolin-1 expression in malignant melanoma metastases predicts poor survival. In: Cell Cycle. 2011 ; Vol. 10, No. 24. pp. 4250-4255.
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