LOVTRAP: An optogenetic system for photoinduced protein dissociation

Hui Wang, Marco Vilela, Andreas Winkler, Miroslaw Tarnawski, Ilme Schlichting, Hayretin Yumerefendi, Brian Kuhlman, Rihe Liu, Gaudenz Danuser, Klaus M. Hahn

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (K d). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.

Original languageEnglish (US)
Pages (from-to)755-758
Number of pages4
JournalNature Methods
Volume13
Issue number9
DOIs
StatePublished - Aug 30 2016

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Optogenetics
rac1 GTP-Binding Protein
Proteins
Staphylococcal Protein A
Phosphatidylinositol 3-Kinases
Mitochondria
Light
Kinetics

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Wang, H., Vilela, M., Winkler, A., Tarnawski, M., Schlichting, I., Yumerefendi, H., ... Hahn, K. M. (2016). LOVTRAP: An optogenetic system for photoinduced protein dissociation. Nature Methods, 13(9), 755-758. https://doi.org/10.1038/nmeth.3926

LOVTRAP : An optogenetic system for photoinduced protein dissociation. / Wang, Hui; Vilela, Marco; Winkler, Andreas; Tarnawski, Miroslaw; Schlichting, Ilme; Yumerefendi, Hayretin; Kuhlman, Brian; Liu, Rihe; Danuser, Gaudenz; Hahn, Klaus M.

In: Nature Methods, Vol. 13, No. 9, 30.08.2016, p. 755-758.

Research output: Contribution to journalArticle

Wang, H, Vilela, M, Winkler, A, Tarnawski, M, Schlichting, I, Yumerefendi, H, Kuhlman, B, Liu, R, Danuser, G & Hahn, KM 2016, 'LOVTRAP: An optogenetic system for photoinduced protein dissociation', Nature Methods, vol. 13, no. 9, pp. 755-758. https://doi.org/10.1038/nmeth.3926
Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi H et al. LOVTRAP: An optogenetic system for photoinduced protein dissociation. Nature Methods. 2016 Aug 30;13(9):755-758. https://doi.org/10.1038/nmeth.3926
Wang, Hui ; Vilela, Marco ; Winkler, Andreas ; Tarnawski, Miroslaw ; Schlichting, Ilme ; Yumerefendi, Hayretin ; Kuhlman, Brian ; Liu, Rihe ; Danuser, Gaudenz ; Hahn, Klaus M. / LOVTRAP : An optogenetic system for photoinduced protein dissociation. In: Nature Methods. 2016 ; Vol. 13, No. 9. pp. 755-758.
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