LOVTRAP

An optogenetic system for photoinduced protein dissociation

Hui Wang, Marco Vilela, Andreas Winkler, Miroslaw Tarnawski, Ilme Schlichting, Hayretin Yumerefendi, Brian Kuhlman, Rihe Liu, Gaudenz Danuser, Klaus M. Hahn

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (K d). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.

Original languageEnglish (US)
Pages (from-to)755-758
Number of pages4
JournalNature Methods
Volume13
Issue number9
DOIs
StatePublished - Aug 30 2016

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Optogenetics
rac1 GTP-Binding Protein
Proteins
Staphylococcal Protein A
Phosphatidylinositol 3-Kinases
Mitochondria
Light
Kinetics

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Wang, H., Vilela, M., Winkler, A., Tarnawski, M., Schlichting, I., Yumerefendi, H., ... Hahn, K. M. (2016). LOVTRAP: An optogenetic system for photoinduced protein dissociation. Nature Methods, 13(9), 755-758. https://doi.org/10.1038/nmeth.3926

LOVTRAP : An optogenetic system for photoinduced protein dissociation. / Wang, Hui; Vilela, Marco; Winkler, Andreas; Tarnawski, Miroslaw; Schlichting, Ilme; Yumerefendi, Hayretin; Kuhlman, Brian; Liu, Rihe; Danuser, Gaudenz; Hahn, Klaus M.

In: Nature Methods, Vol. 13, No. 9, 30.08.2016, p. 755-758.

Research output: Contribution to journalArticle

Wang, H, Vilela, M, Winkler, A, Tarnawski, M, Schlichting, I, Yumerefendi, H, Kuhlman, B, Liu, R, Danuser, G & Hahn, KM 2016, 'LOVTRAP: An optogenetic system for photoinduced protein dissociation', Nature Methods, vol. 13, no. 9, pp. 755-758. https://doi.org/10.1038/nmeth.3926
Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi H et al. LOVTRAP: An optogenetic system for photoinduced protein dissociation. Nature Methods. 2016 Aug 30;13(9):755-758. https://doi.org/10.1038/nmeth.3926
Wang, Hui ; Vilela, Marco ; Winkler, Andreas ; Tarnawski, Miroslaw ; Schlichting, Ilme ; Yumerefendi, Hayretin ; Kuhlman, Brian ; Liu, Rihe ; Danuser, Gaudenz ; Hahn, Klaus M. / LOVTRAP : An optogenetic system for photoinduced protein dissociation. In: Nature Methods. 2016 ; Vol. 13, No. 9. pp. 755-758.
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