LOVTRAP: An optogenetic system for photoinduced protein dissociation

Hui Wang, Marco Vilela, Andreas Winkler, Miroslaw Tarnawski, Ilme Schlichting, Hayretin Yumerefendi, Brian Kuhlman, Rihe Liu, Gaudenz Danuser, Klaus M. Hahn

Research output: Contribution to journalArticlepeer-review

110 Scopus citations

Abstract

LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (K d). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.

Original languageEnglish (US)
Pages (from-to)755-758
Number of pages4
JournalNature methods
Volume13
Issue number9
DOIs
StatePublished - Aug 30 2016

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'LOVTRAP: An optogenetic system for photoinduced protein dissociation'. Together they form a unique fingerprint.

Cite this