LOVTRAP: An optogenetic system for photoinduced protein dissociation

Hui Wang, Marco Vilela, Andreas Winkler, Miroslaw Tarnawski, Ilme Schlichting, Hayretin Yumerefendi, Brian Kuhlman, Rihe Liu, Gaudenz Danuser, Klaus M. Hahn

Research output: Contribution to journalArticle

95 Scopus citations

Abstract

LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (K d). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.

Original languageEnglish (US)
Pages (from-to)755-758
Number of pages4
JournalNature methods
Volume13
Issue number9
DOIs
StatePublished - Aug 30 2016

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Wang, H., Vilela, M., Winkler, A., Tarnawski, M., Schlichting, I., Yumerefendi, H., Kuhlman, B., Liu, R., Danuser, G., & Hahn, K. M. (2016). LOVTRAP: An optogenetic system for photoinduced protein dissociation. Nature methods, 13(9), 755-758. https://doi.org/10.1038/nmeth.3926