The binding of [125I]iodoLDL and [125I]iodoHDL to membrane fractions prepared from fresh adrenal tissue of an anencephalic newborn was examined and compared to the binding of these lipoproteins to membrane fractions prepared from normal human fetal adrenal (HFA) tissue. The apparent dissociation constant (KD) for the interaction of [125I]iodoLDL with its binding sites in membrane fractions prepared from HFA tissue of an anencephalic fetus (23.5 μg - ml-1) was similar to that in membranes from normal HFA tissue. However, the binding capacity for [125I]iodoLDL of membrane fractions prepared from adrenal tissue of an anencephalic fetus (600 ng · mg-1 protein) was only one-third of the binding capacity (1500 ng · mg-1 protein) of normal HFA membrane fractions. On the other hand, the binding capacity for [125I]iodoHDL of membrane fractions prepared from anencephalic and normal adrenal tissue was similar.
- fetal adrenal tissue
- low density lipoprotein
ASJC Scopus subject areas
- Pediatrics, Perinatology, and Child Health