Low density lipoprotein receptors in adrenal tissue of a human anencephalic fetus

Masao Ohashi; Bruce R. Carr; Evan R. Simpson

doi:10.1016/0378-3782(82)90133-5

Low density lipoprotein receptors in adrenal tissue of a human anencephalic fetus

Masao Ohashi, Bruce R. Carr, Evan R. Simpson

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The binding of [¹²⁵I]iodoLDL and [¹²⁵I]iodoHDL to membrane fractions prepared from fresh adrenal tissue of an anencephalic newborn was examined and compared to the binding of these lipoproteins to membrane fractions prepared from normal human fetal adrenal (HFA) tissue. The apparent dissociation constant (K_D) for the interaction of [¹²⁵I]iodoLDL with its binding sites in membrane fractions prepared from HFA tissue of an anencephalic fetus (23.5 μg - ml^-1) was similar to that in membranes from normal HFA tissue. However, the binding capacity for [¹²⁵I]iodoLDL of membrane fractions prepared from adrenal tissue of an anencephalic fetus (600 ng · mg^-1 protein) was only one-third of the binding capacity (1500 ng · mg^-1 protein) of normal HFA membrane fractions. On the other hand, the binding capacity for [¹²⁵I]iodoHDL of membrane fractions prepared from anencephalic and normal adrenal tissue was similar.

Original language	English (US)
Pages (from-to)	149-154
Number of pages	6
Journal	Early Human Development
Volume	7
Issue number	2
DOIs	https://doi.org/10.1016/0378-3782(82)90133-5
State	Published - Nov 1982

Keywords

anencephalic
fetal adrenal tissue
low density lipoprotein

ASJC Scopus subject areas

Pediatrics, Perinatology, and Child Health
Obstetrics and Gynecology

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10.1016/0378-3782(82)90133-5

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title = "Low density lipoprotein receptors in adrenal tissue of a human anencephalic fetus",

abstract = "The binding of [125I]iodoLDL and [125I]iodoHDL to membrane fractions prepared from fresh adrenal tissue of an anencephalic newborn was examined and compared to the binding of these lipoproteins to membrane fractions prepared from normal human fetal adrenal (HFA) tissue. The apparent dissociation constant (KD) for the interaction of [125I]iodoLDL with its binding sites in membrane fractions prepared from HFA tissue of an anencephalic fetus (23.5 μg - ml-1) was similar to that in membranes from normal HFA tissue. However, the binding capacity for [125I]iodoLDL of membrane fractions prepared from adrenal tissue of an anencephalic fetus (600 ng · mg-1 protein) was only one-third of the binding capacity (1500 ng · mg-1 protein) of normal HFA membrane fractions. On the other hand, the binding capacity for [125I]iodoHDL of membrane fractions prepared from anencephalic and normal adrenal tissue was similar.",

keywords = "anencephalic, fetal adrenal tissue, low density lipoprotein",

author = "Masao Ohashi and Carr, {Bruce R.} and Simpson, {Evan R.}",

note = "Funding Information: * Supported, in part, by USPHS Grant Nos. HD13234 and HDlll49. ** Supported. in part, by a grant-in-aid from the Chilton Foundation. Add+ess correspondence to: Bruce R. Carr. M.D., Department of Obstetrics University of Texas Southwestern Medical School, 5323 Harry Hines Boulevard, U.S.A.",

year = "1982",

month = nov,

doi = "10.1016/0378-3782(82)90133-5",

language = "English (US)",

volume = "7",

pages = "149--154",

journal = "Early Human Development",

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TY - JOUR

T1 - Low density lipoprotein receptors in adrenal tissue of a human anencephalic fetus

AU - Ohashi, Masao

AU - Carr, Bruce R.

AU - Simpson, Evan R.

N1 - Funding Information: * Supported, in part, by USPHS Grant Nos. HD13234 and HDlll49. ** Supported. in part, by a grant-in-aid from the Chilton Foundation. Add+ess correspondence to: Bruce R. Carr. M.D., Department of Obstetrics University of Texas Southwestern Medical School, 5323 Harry Hines Boulevard, U.S.A.

PY - 1982/11

Y1 - 1982/11

N2 - The binding of [125I]iodoLDL and [125I]iodoHDL to membrane fractions prepared from fresh adrenal tissue of an anencephalic newborn was examined and compared to the binding of these lipoproteins to membrane fractions prepared from normal human fetal adrenal (HFA) tissue. The apparent dissociation constant (KD) for the interaction of [125I]iodoLDL with its binding sites in membrane fractions prepared from HFA tissue of an anencephalic fetus (23.5 μg - ml-1) was similar to that in membranes from normal HFA tissue. However, the binding capacity for [125I]iodoLDL of membrane fractions prepared from adrenal tissue of an anencephalic fetus (600 ng · mg-1 protein) was only one-third of the binding capacity (1500 ng · mg-1 protein) of normal HFA membrane fractions. On the other hand, the binding capacity for [125I]iodoHDL of membrane fractions prepared from anencephalic and normal adrenal tissue was similar.

AB - The binding of [125I]iodoLDL and [125I]iodoHDL to membrane fractions prepared from fresh adrenal tissue of an anencephalic newborn was examined and compared to the binding of these lipoproteins to membrane fractions prepared from normal human fetal adrenal (HFA) tissue. The apparent dissociation constant (KD) for the interaction of [125I]iodoLDL with its binding sites in membrane fractions prepared from HFA tissue of an anencephalic fetus (23.5 μg - ml-1) was similar to that in membranes from normal HFA tissue. However, the binding capacity for [125I]iodoLDL of membrane fractions prepared from adrenal tissue of an anencephalic fetus (600 ng · mg-1 protein) was only one-third of the binding capacity (1500 ng · mg-1 protein) of normal HFA membrane fractions. On the other hand, the binding capacity for [125I]iodoHDL of membrane fractions prepared from anencephalic and normal adrenal tissue was similar.

KW - anencephalic

KW - fetal adrenal tissue

KW - low density lipoprotein

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ER -

Low density lipoprotein receptors in adrenal tissue of a human anencephalic fetus

Abstract

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