Low dopamine transporter mRNA levels in midbrain regions containing calbindin

M. K. Sanghera, K. F. Manaye, C. L. Liang, A. M. Iacopino, N. J. Bannon, D. C. German

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The dopamine transporter (DAT) is the site at which the neurotoxic metabolite of MPTP gains access to midbrain dopaminergic (DA) neurons. However, not all midbrain DA neurons degenerate following MPTP treatment. The midbrain DA neurons that contain the calcium-binding protein, calbindin-D(28k) (CALB), are relatively invulnerable to MPTP toxicity, compared with DA neurons that lack CALB. Using in situ hybridization and immunocytochemical staining techniques in the rat and mouse, we now report that there is as much as 10 fold less DAT mRNA in regions where DA neurons contain CALB compared with regions where DA neurons lack CALB. These data suggest that specific midbrain DA neurons are invulnerable to MPTP toxicity not only because they contain CALB, but also because they have relatively low DAT activity.

Original languageEnglish (US)
Pages (from-to)1641-1644
Number of pages4
JournalNeuroReport
Volume5
Issue number13
StatePublished - 1994

Fingerprint

Calbindins
Dopamine Plasma Membrane Transport Proteins
Dopaminergic Neurons
Mesencephalon
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
Messenger RNA
Calcium-Binding Proteins
In Situ Hybridization
Staining and Labeling

Keywords

  • Calbindin-D(28k) (CALB)
  • Human dopamine receptor (DAT)
  • Immunocytochemistry
  • In situ hybridization
  • Midbrain dopaminergic (DA) neurons

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Sanghera, M. K., Manaye, K. F., Liang, C. L., Iacopino, A. M., Bannon, N. J., & German, D. C. (1994). Low dopamine transporter mRNA levels in midbrain regions containing calbindin. NeuroReport, 5(13), 1641-1644.

Low dopamine transporter mRNA levels in midbrain regions containing calbindin. / Sanghera, M. K.; Manaye, K. F.; Liang, C. L.; Iacopino, A. M.; Bannon, N. J.; German, D. C.

In: NeuroReport, Vol. 5, No. 13, 1994, p. 1641-1644.

Research output: Contribution to journalArticle

Sanghera, MK, Manaye, KF, Liang, CL, Iacopino, AM, Bannon, NJ & German, DC 1994, 'Low dopamine transporter mRNA levels in midbrain regions containing calbindin', NeuroReport, vol. 5, no. 13, pp. 1641-1644.
Sanghera MK, Manaye KF, Liang CL, Iacopino AM, Bannon NJ, German DC. Low dopamine transporter mRNA levels in midbrain regions containing calbindin. NeuroReport. 1994;5(13):1641-1644.
Sanghera, M. K. ; Manaye, K. F. ; Liang, C. L. ; Iacopino, A. M. ; Bannon, N. J. ; German, D. C. / Low dopamine transporter mRNA levels in midbrain regions containing calbindin. In: NeuroReport. 1994 ; Vol. 5, No. 13. pp. 1641-1644.
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