Lung protection by inhalation of exogenous solubilized extracellular matrix

Jinglei Wu, Priya Ravikumar, Kytai T. Nguyen, Connie C W Hsia, Yi Hong

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Decellularized extracellular matrix (ECM) contains complex tissue-specific components that work in concert to promote tissue repair and constructive remodeling and has been used experimentally and clinically to accelerate epithelial wound repair, leading us to hypothesize that lung-derived ECM could mitigate acute lung injury. To explore the therapeutic potential of ECM for noninvasive delivery to the lung, we decellularized and solubilized porcine lung ECM, then characterized the composition, concentration, particle size and stability of the preparation. The ECM preparation at 3.2 mg/mL with average particle size <3 μm was tested in vitro on human A549 lung epithelial cells exposed to 95% O2 for 24 hours, and in vivo by tracheal instillation or nebulization into the lungs of rats exposed intermittently or continuously to 90% O2 for a cumulative 72 hours. Our results showed that the preparation was enriched in collagen, reduced in glycosaminoglycans, and contained various bioactive molecules. Particle size was concentration-dependent. Compared to the respective controls treated with cell culture medium in vitro or saline in vivo, ECM inhalation normalized cell survival and alveolar morphology, and reduced hyperoxia-induced apoptosis and oxidative damage. This proof-of-concept study established the methodology, feasibility and therapeutic potential of exogenous solubilized ECM for pulmonary cytoprotection, possibly as an adjunct or potentiator of conventional therapy.

Original languageEnglish (US)
Article numbere0171165
JournalPLoS One
Volume12
Issue number2
DOIs
StatePublished - Feb 1 2017

Fingerprint

extracellular matrix
Inhalation
breathing
Extracellular Matrix
lungs
Particle size
Lung
Repair
Tissue
Particle Size
particle size
Glycosaminoglycans
Cell culture
Culture Media
Rats
therapeutics
Collagen
Cells
Apoptosis
Molecules

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Lung protection by inhalation of exogenous solubilized extracellular matrix. / Wu, Jinglei; Ravikumar, Priya; Nguyen, Kytai T.; Hsia, Connie C W; Hong, Yi.

In: PLoS One, Vol. 12, No. 2, e0171165, 01.02.2017.

Research output: Contribution to journalArticle

Wu, Jinglei ; Ravikumar, Priya ; Nguyen, Kytai T. ; Hsia, Connie C W ; Hong, Yi. / Lung protection by inhalation of exogenous solubilized extracellular matrix. In: PLoS One. 2017 ; Vol. 12, No. 2.
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