Lung surfactant microbubbles

Shashank Sirsi, Calvin Pae, David Kyung Taek Oh, Henning Blomback, Adel Koubaa, Brigitte Papahadjopoulos-Sternberg, Mark Borden

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The observation of stable microbubbles was instrumental to the discovery of lung surfactant and has been turned into a clinical tool for the prediction of neonatal respiratory distress syndrome. However, the colloidal properties of lung surfactant microbubbles have not been well characterized prior to this study. We show here that a common exogenous lung surfactant, Survanta, is capable of routinely stabilizing approximately a billion microbubbles per mL, most of which are less than 10 m diameter, when perfluorobutane was used as the filling gas. The formulation therefore has remarkably rapid adsorption kinetics and surface tension lowering ability. The addition of an emulsifier did not significantly enhance microbubble production. Fluorescence microscopy showed prominent collapse structures on these microbubbles, and freeze-fracture electron microscopy showed undulation patterns of the monolayer surface. Zeta potential analysis indicated that Survanta microbubbles were highly negatively charged. Quantitative measurements of microbubble production allowed an analysis of competitive adsorption. Bovine serum albumin inhibited Survanta adsorption in a dose-response manner. Microbubble production was not reactivated by the addition of the nonionic polymer, poly(ethylene glycol). Lung surfactant microbubbles can serve as a tool to evaluate surfactant function and, potentially, as a theranostic agent for ultrasound.

Original languageEnglish (US)
Pages (from-to)4835-4842
Number of pages8
JournalSoft Matter
Volume5
Issue number23
DOIs
StatePublished - Nov 30 2009

Fingerprint

Surface-Active Agents
lungs
surfactants
Adsorption
adsorption
Fluorescence microscopy
Zeta potential
Bovine Serum Albumin
albumins
serums
Electron microscopy
Polyethylene glycols
Surface tension
glycols
Monolayers
electron microscopy
Polymers
interfacial tension
ethylene
Gases

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics

Cite this

Sirsi, S., Pae, C., Taek Oh, D. K., Blomback, H., Koubaa, A., Papahadjopoulos-Sternberg, B., & Borden, M. (2009). Lung surfactant microbubbles. Soft Matter, 5(23), 4835-4842. https://doi.org/10.1039/b915065j

Lung surfactant microbubbles. / Sirsi, Shashank; Pae, Calvin; Taek Oh, David Kyung; Blomback, Henning; Koubaa, Adel; Papahadjopoulos-Sternberg, Brigitte; Borden, Mark.

In: Soft Matter, Vol. 5, No. 23, 30.11.2009, p. 4835-4842.

Research output: Contribution to journalArticle

Sirsi, S, Pae, C, Taek Oh, DK, Blomback, H, Koubaa, A, Papahadjopoulos-Sternberg, B & Borden, M 2009, 'Lung surfactant microbubbles', Soft Matter, vol. 5, no. 23, pp. 4835-4842. https://doi.org/10.1039/b915065j
Sirsi S, Pae C, Taek Oh DK, Blomback H, Koubaa A, Papahadjopoulos-Sternberg B et al. Lung surfactant microbubbles. Soft Matter. 2009 Nov 30;5(23):4835-4842. https://doi.org/10.1039/b915065j
Sirsi, Shashank ; Pae, Calvin ; Taek Oh, David Kyung ; Blomback, Henning ; Koubaa, Adel ; Papahadjopoulos-Sternberg, Brigitte ; Borden, Mark. / Lung surfactant microbubbles. In: Soft Matter. 2009 ; Vol. 5, No. 23. pp. 4835-4842.
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