Lysine propionylation and butyrylation are novel post-translational modifications in histones

Yue Chen, Robert Sprung, Yi Tang, Haydn L Ball, Bhavani Sangras, Sung Chan Kim, J R Falck, Junmin Peng, Wei Gu, Yingming Zhao

Research output: Contribution to journalArticle

329 Citations (Scopus)

Abstract

The positively charged lysine residue plays an important role in protein folding and functions. Neutralization of the charge often has a profound impact on the substrate proteins. Accordingly all the known post-translational modifications at lysine have pivotal roles in cell physiology and pathology. Here we report the discovery of two novel, in vivo lysine modifications in histones, lysine propionylation and butyrylation. We confirmed, by in vitro labeling and peptide mapping by mass spectrometry, that two previously known acetyltransferases, p3OO and CREB-binding protein, could catalyze lysine propionylation and lysine butyrylation in histones. Finally p300 and CREB-binding protein could carry out autopropionylation and autobutyrylation in vitro. Taken together, our results conclusively establish that lysine propionylation and lysine butyrylation are novel post-translational modifications. Given the unique roles of propionyl-CoA and butyryl-CoA in energy metabolism and the significant structural changes induced by the modifications, the two modifications are likely to have important but distinct functions in the regulation of biological processes.

Original languageEnglish (US)
Pages (from-to)812-819
Number of pages8
JournalMolecular and Cellular Proteomics
Volume6
Issue number5
DOIs
StatePublished - May 2007

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Post Translational Protein Processing
Histones
Lysine
CREB-Binding Protein
Histone Code
Biological Phenomena
Protein folding
Cell Physiological Phenomena
Acetyltransferases
Peptide Mapping
Protein Folding
Physiology
Pathology
Labeling
Energy Metabolism
Mass spectrometry
Mass Spectrometry
Peptides
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Lysine propionylation and butyrylation are novel post-translational modifications in histones. / Chen, Yue; Sprung, Robert; Tang, Yi; Ball, Haydn L; Sangras, Bhavani; Kim, Sung Chan; Falck, J R; Peng, Junmin; Gu, Wei; Zhao, Yingming.

In: Molecular and Cellular Proteomics, Vol. 6, No. 5, 05.2007, p. 812-819.

Research output: Contribution to journalArticle

Chen, Yue ; Sprung, Robert ; Tang, Yi ; Ball, Haydn L ; Sangras, Bhavani ; Kim, Sung Chan ; Falck, J R ; Peng, Junmin ; Gu, Wei ; Zhao, Yingming. / Lysine propionylation and butyrylation are novel post-translational modifications in histones. In: Molecular and Cellular Proteomics. 2007 ; Vol. 6, No. 5. pp. 812-819.
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AU - Peng, Junmin

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