Lysines 3241 and 3260 of DNA-PKcs are important for genomic stability and radioresistance

Eiichiro Mori, Anthony J. Davis, Masatoshi Hasegawa, David J. Chen

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

DNA-dependent protein kinase (DNA-PK) is a serine/threonine kinase that plays an essential role in the repair of DNA double-strand breaks (DSBs) in the non-homologous end-joining (NHEJ) pathway. The DNA-PK holoenzyme consists of a catalytic subunit (DNA-PKcs) and DNA-binding subunit (Ku70/80, Ku). Ku is a molecular sensor for double-stranded DNA and once bound to DSB ends it recruits DNA-PKcs to the DSB site. Subsequently, DNA-PKcs is activated and heavily phosphorylated, with these phosphorylations modulating DNA-PKcs. Although phosphorylation of DNA-PKcs is well studied, other post-translational modifications of DNA-PKcs are not. In this study, we aimed to determine if acetylation of DNA-PKcs regulates DNA-PKcs-dependent DSB repair. We report that DNA-PKcs is acetylated in vivo and identified two putative acetylation sites, lysine residues 3241 and 3260. Mutating these sites to block potential acetylation results in increased radiosensitive, a slight decrease in DSB repair capacity as assessed by γH2AX resolution, and increased chromosomal aberrations, especially quadriradial chromosomes. Together, our results provide evidence that acetylation potentially regulates DNA-PKcs.

Original languageEnglish (US)
Pages (from-to)235-240
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume477
Issue number2
DOIs
StatePublished - Aug 19 2016

Keywords

  • Acetylation
  • DNA double-strand breaks
  • DNA-PKcs
  • Non-homologous end-joining

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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