Lysosomal alterations in autolyzing rabbit heart

Robert S. Decker, A. Robin Poole, J. T. Dingle, Kern Wildenthal

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Sequential biochemical and immunohistochemical lysosomal alterations were assessed in autolyzing ventricular myocardium incubated at 37°C in a humidified atmosphere for 15 min to 2 h. A profound redistribution of cathepsin D activity into the nonsedimentable fraction of homogenates could be demonstrated after only 15 min of autolysis, with further increases over the next 105 min. Nonsedimentable activity of n-acetyl-β,d-glucosaminidase was also significantly increased early after the onset of autolysis, but the distribution of acid phosphatase activity was not significantly altered. Immunohistochemical localization of cathepsin D revealed dramatic loss of granular staining in myocytes during the first hour of autolysis. Between 15 and 30 min, lysosomes became enlarged and halos of fluorescent material appeared, representing diffusion of cathepsin D from lysosomes into the myoplasm. By 60 min most cathepsin-D-positive organelles had vanished and the enzyme was instead distributed diffusely throughout the cell. The early redistribution of cathepsin D observed by biochemical and immunohistochemical techniques occurred before electron microscopic indications of cell death became apparent. Thus, autolyzing heart may provide a useful model for studying the possible role of lysosomal enzymes in provoking myocardial cell injury and necrosis.

Original languageEnglish (US)
JournalJournal of Molecular and Cellular Cardiology
Volume11
Issue number2
DOIs
StatePublished - 1979

Fingerprint

Cathepsin D
Autolysis
Rabbits
Lysosomes
Hexosaminidases
Enzymes
Acid Phosphatase
Atmosphere
Organelles
Muscle Cells
Myocardium
Cell Death
Necrosis
Electrons
Staining and Labeling
Wounds and Injuries

Keywords

  • Acid phosphatase
  • Cardiac necrosis
  • Cathepsin D
  • Electron microscopy
  • Immunohistochemistry
  • n-acetyl-β,d-glucosaminidase

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Lysosomal alterations in autolyzing rabbit heart. / Decker, Robert S.; Poole, A. Robin; Dingle, J. T.; Wildenthal, Kern.

In: Journal of Molecular and Cellular Cardiology, Vol. 11, No. 2, 1979.

Research output: Contribution to journalArticle

Decker, Robert S. ; Poole, A. Robin ; Dingle, J. T. ; Wildenthal, Kern. / Lysosomal alterations in autolyzing rabbit heart. In: Journal of Molecular and Cellular Cardiology. 1979 ; Vol. 11, No. 2.
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