Lysosomal alterations in hypoxic and reoxygenated hearts. II. Immunohistochemical and biochemical changes in cathepsin D

R. S. Decker, A. R. Poole, J. S. Crie, J. T. Dingle, K. Wildenthal

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40 Scopus citations

Abstract

Sublethal hypoxic injury in rat and rabbit hearts was accompanied by a biochemical redistribution of cathepsin D activity from the particle to the supernatant fraction of the tissue homogenate, which was partially reversible on reoxygenation. Immunofluorescent staining for cathepsin D failed to reveal major anatomic release of the acid hydrolase until necrosis was present, suggesting that the earlier biochemical redistribution was primarily a result of increased lysosomal fragility during homogenization, with significant intracellular diffusion of the enzyme occurring only as irreversible damage took place. Hypoxia produced enlargement of both cathepsin-D-staining lysosomes and nonstaining vacuoles, as well as their aggregation. These changes were intensified during reoxygenation and recovery of reversibly damaged hearts, suggesting a possible role for the lysosomal-vacuolar apparatus in myocytic repair following hypoxic injury.

Original languageEnglish (US)
Pages (from-to)445-455
Number of pages11
JournalAmerican Journal of Pathology
Volume98
Issue number2
StatePublished - Jul 25 1980

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Decker, R. S., Poole, A. R., Crie, J. S., Dingle, J. T., & Wildenthal, K. (1980). Lysosomal alterations in hypoxic and reoxygenated hearts. II. Immunohistochemical and biochemical changes in cathepsin D. American Journal of Pathology, 98(2), 445-455.