Macrophage fatty acid composition and phagocytosis

Effect of unsaturation on cellular phagocytic activity

A. J. Schroit, R. Gallily

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

In order to manipulate the physical properties of the macrophage membrane, methods were developed which potentiated the incorporation of exogenously supplied fatty acids into membrane lipids. Chromatograms of macrophages which were grown in the presence of a variety of fatty acids demonstrated that exogenously supplied unsaturated fatty acids (palmitoleic, oleic, elaidic, linoleic, linolenic and arachidonic acids) were readily incorporated into the cells and selectively altered the fatty acyl composition of macrophage phospholipids. Up to 38% of the total cellular phospholipids were found to be derived from the exogenously added fatty acid supplements. The incorporation of the different fatty acids into cellular phospholipids had striking effects on cellular phagocytic activity. These effects were found to correlate with the degree of unsaturation, and the cis- or trans-double bond configuration. Thus, macrophage phagocytic ingestion rates of 125I-labelled Shigella flexneri were found to alter by more than 2-fold after the cells were cultivated in the presence of cis unsaturated fatty acids.

Original languageEnglish (US)
Pages (from-to)199-205
Number of pages7
JournalImmunology
Volume36
Issue number2
StatePublished - 1979

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Phagocytosis
Fatty Acids
Macrophages
Phospholipids
Unsaturated Fatty Acids
Linolenic Acids
Oleic Acids
Linoleic Acids
Arachidonic Acids
Shigella flexneri
Membrane Lipids
Eating
Membranes

ASJC Scopus subject areas

  • Immunology

Cite this

Macrophage fatty acid composition and phagocytosis : Effect of unsaturation on cellular phagocytic activity. / Schroit, A. J.; Gallily, R.

In: Immunology, Vol. 36, No. 2, 1979, p. 199-205.

Research output: Contribution to journalArticle

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