Macrophages induce differentiation of plasma cells through CXCL10/IP-10

Wei Xu; HyeMee Joo; Sandra Clayton; Melissa Dullaers; Marie Cecile Herve; Derek Blankenship; Maria Teresa De La Morena; Robert Balderas; Capucine Picard; Jean Laurent Casanova; Virginia Pascual; SangKon Oh; Jacques Banchereau

doi:10.1084/jem.20112142

Macrophages induce differentiation of plasma cells through CXCL10/IP-10

Wei Xu, HyeMee Joo, Sandra Clayton, Melissa Dullaers, Marie Cecile Herve, Derek Blankenship, Maria Teresa De La Morena, Robert Balderas, Capucine Picard, Jean Laurent Casanova, Virginia Pascual, SangKon Oh, Jacques Banchereau

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

In tonsils, CD138⁺ plasma cells (PCs) are surrounded by CD163⁺ resident macrophages (Mφs). We show here that human Mφs (isolated from tonsils or generated from monocytes in vitro) drive activated B cells to differentiate into CD138⁺CD38⁺⁺ PCs through secreted CXCL10/IP-10 and VCAM-1 contact. IP-10 production by Mφs is induced by B cell-derived IL-6 and depends on STAT3 phosphorylation. Furthermore, IP-10 amplifies the production of IL-6 by B cells, which sustains the STAT3 signals that lead to PC differentiation. IP-10- deficient mice challenged with NP-Ficoll show a decreased frequency of NP-specific PCs and lower titers of antibodies. Thus, our results reveal a novel dialog between Mφs and B cells, in which IP-10 acts as a PC differentiation factor.

Original language	English (US)
Pages (from-to)	1813-1823
Number of pages	11
Journal	Journal of Experimental Medicine
Volume	209
Issue number	10
DOIs	https://doi.org/10.1084/jem.20112142
State	Published - Sep 2012

ASJC Scopus subject areas

General Medicine

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10.1084/jem.20112142

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@article{9760282372c346c99712eee3d4285dc2,

title = "Macrophages induce differentiation of plasma cells through CXCL10/IP-10",

abstract = "In tonsils, CD138+ plasma cells (PCs) are surrounded by CD163+ resident macrophages (Mφs). We show here that human Mφs (isolated from tonsils or generated from monocytes in vitro) drive activated B cells to differentiate into CD138+CD38++ PCs through secreted CXCL10/IP-10 and VCAM-1 contact. IP-10 production by Mφs is induced by B cell-derived IL-6 and depends on STAT3 phosphorylation. Furthermore, IP-10 amplifies the production of IL-6 by B cells, which sustains the STAT3 signals that lead to PC differentiation. IP-10- deficient mice challenged with NP-Ficoll show a decreased frequency of NP-specific PCs and lower titers of antibodies. Thus, our results reveal a novel dialog between Mφs and B cells, in which IP-10 acts as a PC differentiation factor.",

author = "Wei Xu and HyeMee Joo and Sandra Clayton and Melissa Dullaers and Herve, {Marie Cecile} and Derek Blankenship and {De La Morena}, {Maria Teresa} and Robert Balderas and Capucine Picard and Casanova, {Jean Laurent} and Virginia Pascual and SangKon Oh and Jacques Banchereau",

year = "2012",

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doi = "10.1084/jem.20112142",

language = "English (US)",

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T1 - Macrophages induce differentiation of plasma cells through CXCL10/IP-10

AU - Xu, Wei

AU - Joo, HyeMee

AU - Clayton, Sandra

AU - Dullaers, Melissa

AU - Herve, Marie Cecile

AU - Blankenship, Derek

AU - De La Morena, Maria Teresa

AU - Balderas, Robert

AU - Picard, Capucine

AU - Casanova, Jean Laurent

AU - Pascual, Virginia

AU - Oh, SangKon

AU - Banchereau, Jacques

PY - 2012/9

Y1 - 2012/9

N2 - In tonsils, CD138+ plasma cells (PCs) are surrounded by CD163+ resident macrophages (Mφs). We show here that human Mφs (isolated from tonsils or generated from monocytes in vitro) drive activated B cells to differentiate into CD138+CD38++ PCs through secreted CXCL10/IP-10 and VCAM-1 contact. IP-10 production by Mφs is induced by B cell-derived IL-6 and depends on STAT3 phosphorylation. Furthermore, IP-10 amplifies the production of IL-6 by B cells, which sustains the STAT3 signals that lead to PC differentiation. IP-10- deficient mice challenged with NP-Ficoll show a decreased frequency of NP-specific PCs and lower titers of antibodies. Thus, our results reveal a novel dialog between Mφs and B cells, in which IP-10 acts as a PC differentiation factor.

AB - In tonsils, CD138+ plasma cells (PCs) are surrounded by CD163+ resident macrophages (Mφs). We show here that human Mφs (isolated from tonsils or generated from monocytes in vitro) drive activated B cells to differentiate into CD138+CD38++ PCs through secreted CXCL10/IP-10 and VCAM-1 contact. IP-10 production by Mφs is induced by B cell-derived IL-6 and depends on STAT3 phosphorylation. Furthermore, IP-10 amplifies the production of IL-6 by B cells, which sustains the STAT3 signals that lead to PC differentiation. IP-10- deficient mice challenged with NP-Ficoll show a decreased frequency of NP-specific PCs and lower titers of antibodies. Thus, our results reveal a novel dialog between Mφs and B cells, in which IP-10 acts as a PC differentiation factor.

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JF - Journal of Experimental Medicine

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Macrophages induce differentiation of plasma cells through CXCL10/IP-10

Abstract

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