Macrophages induce differentiation of plasma cells through CXCL10/IP-10

Wei Xu, HyeMee Joo, Sandra Clayton, Melissa Dullaers, Marie Cecile Herve, Derek Blankenship, Maria Teresa De La Morena, Robert Balderas, Capucine Picard, Jean Laurent Casanova, Virginia Pascual, SangKon Oh, Jacques Banchereau

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

In tonsils, CD138+ plasma cells (PCs) are surrounded by CD163+ resident macrophages (Mφs). We show here that human Mφs (isolated from tonsils or generated from monocytes in vitro) drive activated B cells to differentiate into CD138+CD38++ PCs through secreted CXCL10/IP-10 and VCAM-1 contact. IP-10 production by Mφs is induced by B cell-derived IL-6 and depends on STAT3 phosphorylation. Furthermore, IP-10 amplifies the production of IL-6 by B cells, which sustains the STAT3 signals that lead to PC differentiation. IP-10- deficient mice challenged with NP-Ficoll show a decreased frequency of NP-specific PCs and lower titers of antibodies. Thus, our results reveal a novel dialog between Mφs and B cells, in which IP-10 acts as a PC differentiation factor.

Original languageEnglish (US)
Pages (from-to)1813-1823
Number of pages11
JournalJournal of Experimental Medicine
Volume209
Issue number10
DOIs
StatePublished - Sep 2012

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Plasma Cells
Macrophages
B-Lymphocytes
Palatine Tonsil
Cell Differentiation
Interleukin-6
Ficoll
Vascular Cell Adhesion Molecule-1
Monocytes
Phosphorylation
Antibodies

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Xu, W., Joo, H., Clayton, S., Dullaers, M., Herve, M. C., Blankenship, D., ... Banchereau, J. (2012). Macrophages induce differentiation of plasma cells through CXCL10/IP-10. Journal of Experimental Medicine, 209(10), 1813-1823. https://doi.org/10.1084/jem.20112142

Macrophages induce differentiation of plasma cells through CXCL10/IP-10. / Xu, Wei; Joo, HyeMee; Clayton, Sandra; Dullaers, Melissa; Herve, Marie Cecile; Blankenship, Derek; De La Morena, Maria Teresa; Balderas, Robert; Picard, Capucine; Casanova, Jean Laurent; Pascual, Virginia; Oh, SangKon; Banchereau, Jacques.

In: Journal of Experimental Medicine, Vol. 209, No. 10, 09.2012, p. 1813-1823.

Research output: Contribution to journalArticle

Xu, W, Joo, H, Clayton, S, Dullaers, M, Herve, MC, Blankenship, D, De La Morena, MT, Balderas, R, Picard, C, Casanova, JL, Pascual, V, Oh, S & Banchereau, J 2012, 'Macrophages induce differentiation of plasma cells through CXCL10/IP-10', Journal of Experimental Medicine, vol. 209, no. 10, pp. 1813-1823. https://doi.org/10.1084/jem.20112142
Xu W, Joo H, Clayton S, Dullaers M, Herve MC, Blankenship D et al. Macrophages induce differentiation of plasma cells through CXCL10/IP-10. Journal of Experimental Medicine. 2012 Sep;209(10):1813-1823. https://doi.org/10.1084/jem.20112142
Xu, Wei ; Joo, HyeMee ; Clayton, Sandra ; Dullaers, Melissa ; Herve, Marie Cecile ; Blankenship, Derek ; De La Morena, Maria Teresa ; Balderas, Robert ; Picard, Capucine ; Casanova, Jean Laurent ; Pascual, Virginia ; Oh, SangKon ; Banchereau, Jacques. / Macrophages induce differentiation of plasma cells through CXCL10/IP-10. In: Journal of Experimental Medicine. 2012 ; Vol. 209, No. 10. pp. 1813-1823.
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