Magnetic resonance imaging detects a specific peptide-protein binding event

Luis M. De León-Rodríguez, Alfonso Ortiz, Allison L. Weiner, Shanrong Zhang, Zoltan Kovacs, Thomas Kodadek, A. Dean Sherry

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

DOTA was conjugated to the N-terminus of a 12-mer peptide by using standard peptide synthesis chemistry. The peptide, first isolated by phage display, maintained a high affinity for its protein-binding target, Gal-80, even with GdDOTA attached. The high affinity constant (KA = 5 × 105 M-1) combined with the high relaxivity of the resulting GdDOTA-peptide·protein complex (r1bound = 44.8 ± 1.7 mM-1 s-1) allowed detection of Gal-80 at μM levels using a standard magnetic resonance imaging protocol. This novel peptide-based, binding-activated MRI method could potentially be used to screen a wide variety of biomolecules.

Original languageEnglish (US)
Pages (from-to)3514-3515
Number of pages2
JournalJournal of the American Chemical Society
Volume124
Issue number14
DOIs
StatePublished - Apr 10 2002

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Protein Binding
Magnetic resonance imaging
Peptides
Magnetic Resonance Imaging
Bacteriophages
Biomolecules
Magnetic resonance
Display devices
Imaging techniques

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Magnetic resonance imaging detects a specific peptide-protein binding event. / De León-Rodríguez, Luis M.; Ortiz, Alfonso; Weiner, Allison L.; Zhang, Shanrong; Kovacs, Zoltan; Kodadek, Thomas; Sherry, A. Dean.

In: Journal of the American Chemical Society, Vol. 124, No. 14, 10.04.2002, p. 3514-3515.

Research output: Contribution to journalArticle

De León-Rodríguez, Luis M. ; Ortiz, Alfonso ; Weiner, Allison L. ; Zhang, Shanrong ; Kovacs, Zoltan ; Kodadek, Thomas ; Sherry, A. Dean. / Magnetic resonance imaging detects a specific peptide-protein binding event. In: Journal of the American Chemical Society. 2002 ; Vol. 124, No. 14. pp. 3514-3515.
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