Magnetic resonance spectroscopy of diffuse brain trauma in the pig

Douglas H. Smith, Kim M. Cecil, David F. Meaney, Xiao Han Chen, Tracy K. McIntosh, Thomas A. Gennarelli, Robert E. Lenkinski

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

The acute metabolic events linked to the evolution of selective axonal pathology in the white matter following diffuse brain injury have not previously been evaluated due to the paucity of relevant experimental models. Here, we utilized a new model of inertial brain injury in the pig that selectively damages axons in the white matter, and applied proton and phosphorous magnetic resonance spectroscopy (MRS) to noninvasively monitor the temporal course of metabolic changes following trauma. Evaluating four pigs with MRS prior to injury, within 1 h and 3 and 7 days postinjury, we found that widespread axonal injury was produced in the absence of changes in pH, PCr/Pi, or the concentrations of ATP, and lactate. However, we did observe an acute 60% loss of intracellular Mg2+ levels, which gradually resolved by 7 days postinjury. In addition, we found that the levels of the neuron marker, N-acetylaspartate (NAA), acutely dropped 20% and remained persistently de creased for at least 7 days postinjury. Moreover, the changes in Mg2+ and NAA were found with MRS in the absence of abnormalities with conventional magnetic resonance imaging (MRI). These results show that (1) profound alterations in intracellular metabolism occur acutely following diffuse axonal pathology in the white matter, but in the absence of indicators of ischemia, and (2) axonal pathology may be evaluated with high sensitivity utilizing noninvasive MRS techniques.

Original languageEnglish (US)
Pages (from-to)665-674
Number of pages10
JournalJournal of Neurotrauma
Volume15
Issue number9
StatePublished - Sep 1998

Fingerprint

Magnetic Resonance Spectroscopy
Swine
Pathology
Wounds and Injuries
Brain Injuries
Axons
Lactic Acid
Theoretical Models
Ischemia
Adenosine Triphosphate
Magnetic Resonance Imaging
Neurons
Traumatic Brain Injury
White Matter
N-acetylaspartate
Diffuse Brain Injury
Proton Magnetic Resonance Spectroscopy

Keywords

  • Axonal injury
  • Diffuse brain injury
  • NAA
  • Phosphorus MRS
  • Proton MRS

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Smith, D. H., Cecil, K. M., Meaney, D. F., Chen, X. H., McIntosh, T. K., Gennarelli, T. A., & Lenkinski, R. E. (1998). Magnetic resonance spectroscopy of diffuse brain trauma in the pig. Journal of Neurotrauma, 15(9), 665-674.

Magnetic resonance spectroscopy of diffuse brain trauma in the pig. / Smith, Douglas H.; Cecil, Kim M.; Meaney, David F.; Chen, Xiao Han; McIntosh, Tracy K.; Gennarelli, Thomas A.; Lenkinski, Robert E.

In: Journal of Neurotrauma, Vol. 15, No. 9, 09.1998, p. 665-674.

Research output: Contribution to journalArticle

Smith, DH, Cecil, KM, Meaney, DF, Chen, XH, McIntosh, TK, Gennarelli, TA & Lenkinski, RE 1998, 'Magnetic resonance spectroscopy of diffuse brain trauma in the pig', Journal of Neurotrauma, vol. 15, no. 9, pp. 665-674.
Smith DH, Cecil KM, Meaney DF, Chen XH, McIntosh TK, Gennarelli TA et al. Magnetic resonance spectroscopy of diffuse brain trauma in the pig. Journal of Neurotrauma. 1998 Sep;15(9):665-674.
Smith, Douglas H. ; Cecil, Kim M. ; Meaney, David F. ; Chen, Xiao Han ; McIntosh, Tracy K. ; Gennarelli, Thomas A. ; Lenkinski, Robert E. / Magnetic resonance spectroscopy of diffuse brain trauma in the pig. In: Journal of Neurotrauma. 1998 ; Vol. 15, No. 9. pp. 665-674.
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