Magnetization transfer micro-MR imaging of live excised lamprey spinal cord

Characterization and immunohistochemical correlation

Hidemasa Uematsu, Andra Popescu, Guixin Zhang, Alexander C. Wright, Suzanne L. Wehrli, Masaya Takahashi, Felix W. Wehrli, Michael E. Selzer, David B. Hackney

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

BACKGROUND AND PURPOSE: Membrane constituents may play a key role in the magnetization transfer (MT) effect. In lamprey spinal cord, axonal diameters range from <1 μm in the dorsal region to 20-40 μm in the ventral region. There is a corresponding range of axonal, and hence cell membrane, density. These characteristics permit determination of the effect of cell membrane density on MT. The purpose of this study was to characterize regional MT effects in lamprey spinal cord. METHODS: Excised spinal cords from eight sea lampreys were measured with a 9.4-T MR imaging system. MT saturation was applied for spin-echo sequences. The MT ratio (MTR) was calculated in each location (dorsal, lateral, and ventral columns). Spinal cords from five other lampreys were prepared with an antibody to lamprey glial keratin (LCM 29). The percentage of area staining with LCM29 was calculated for each location. RESULTS: Mean MTR (± SD) for the dorsal, lateral, and ventral columns were 62.4 ± 4.2, 59.2 ± 2.7, and 56.9 ± 3.0, respectively; all differences were significant (P < .05). Mean LCM29-positive areas for the dorsal, lateral, and ventral columns were 85.1%, 69.7%, and 50.9%, respectively. MTR and percentage LCM29-positive area were significantly correlated (r2 = 0.98). CONCLUSION: Regional differences in MT effect exist in the lamprey spinal cord. MTR is well correlated with percentage LCM29-positive area. These results support the hypothesis that membrane constituents are at least partly responsible for regional variations in MT effect.

Original languageEnglish (US)
Pages (from-to)1816-1820
Number of pages5
JournalAmerican Journal of Neuroradiology
Volume25
Issue number10
StatePublished - Nov 2004

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Lampreys
Spinal Cord
Cell Count
Petromyzon
Cell Membrane
Membranes
Keratins
Neuroglia
Staining and Labeling
Antibodies

ASJC Scopus subject areas

  • Clinical Neurology
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Magnetization transfer micro-MR imaging of live excised lamprey spinal cord : Characterization and immunohistochemical correlation. / Uematsu, Hidemasa; Popescu, Andra; Zhang, Guixin; Wright, Alexander C.; Wehrli, Suzanne L.; Takahashi, Masaya; Wehrli, Felix W.; Selzer, Michael E.; Hackney, David B.

In: American Journal of Neuroradiology, Vol. 25, No. 10, 11.2004, p. 1816-1820.

Research output: Contribution to journalArticle

Uematsu, H, Popescu, A, Zhang, G, Wright, AC, Wehrli, SL, Takahashi, M, Wehrli, FW, Selzer, ME & Hackney, DB 2004, 'Magnetization transfer micro-MR imaging of live excised lamprey spinal cord: Characterization and immunohistochemical correlation', American Journal of Neuroradiology, vol. 25, no. 10, pp. 1816-1820.
Uematsu, Hidemasa ; Popescu, Andra ; Zhang, Guixin ; Wright, Alexander C. ; Wehrli, Suzanne L. ; Takahashi, Masaya ; Wehrli, Felix W. ; Selzer, Michael E. ; Hackney, David B. / Magnetization transfer micro-MR imaging of live excised lamprey spinal cord : Characterization and immunohistochemical correlation. In: American Journal of Neuroradiology. 2004 ; Vol. 25, No. 10. pp. 1816-1820.
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abstract = "BACKGROUND AND PURPOSE: Membrane constituents may play a key role in the magnetization transfer (MT) effect. In lamprey spinal cord, axonal diameters range from <1 μm in the dorsal region to 20-40 μm in the ventral region. There is a corresponding range of axonal, and hence cell membrane, density. These characteristics permit determination of the effect of cell membrane density on MT. The purpose of this study was to characterize regional MT effects in lamprey spinal cord. METHODS: Excised spinal cords from eight sea lampreys were measured with a 9.4-T MR imaging system. MT saturation was applied for spin-echo sequences. The MT ratio (MTR) was calculated in each location (dorsal, lateral, and ventral columns). Spinal cords from five other lampreys were prepared with an antibody to lamprey glial keratin (LCM 29). The percentage of area staining with LCM29 was calculated for each location. RESULTS: Mean MTR (± SD) for the dorsal, lateral, and ventral columns were 62.4 ± 4.2, 59.2 ± 2.7, and 56.9 ± 3.0, respectively; all differences were significant (P < .05). Mean LCM29-positive areas for the dorsal, lateral, and ventral columns were 85.1{\%}, 69.7{\%}, and 50.9{\%}, respectively. MTR and percentage LCM29-positive area were significantly correlated (r2 = 0.98). CONCLUSION: Regional differences in MT effect exist in the lamprey spinal cord. MTR is well correlated with percentage LCM29-positive area. These results support the hypothesis that membrane constituents are at least partly responsible for regional variations in MT effect.",
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AU - Uematsu, Hidemasa

AU - Popescu, Andra

AU - Zhang, Guixin

AU - Wright, Alexander C.

AU - Wehrli, Suzanne L.

AU - Takahashi, Masaya

AU - Wehrli, Felix W.

AU - Selzer, Michael E.

AU - Hackney, David B.

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N2 - BACKGROUND AND PURPOSE: Membrane constituents may play a key role in the magnetization transfer (MT) effect. In lamprey spinal cord, axonal diameters range from <1 μm in the dorsal region to 20-40 μm in the ventral region. There is a corresponding range of axonal, and hence cell membrane, density. These characteristics permit determination of the effect of cell membrane density on MT. The purpose of this study was to characterize regional MT effects in lamprey spinal cord. METHODS: Excised spinal cords from eight sea lampreys were measured with a 9.4-T MR imaging system. MT saturation was applied for spin-echo sequences. The MT ratio (MTR) was calculated in each location (dorsal, lateral, and ventral columns). Spinal cords from five other lampreys were prepared with an antibody to lamprey glial keratin (LCM 29). The percentage of area staining with LCM29 was calculated for each location. RESULTS: Mean MTR (± SD) for the dorsal, lateral, and ventral columns were 62.4 ± 4.2, 59.2 ± 2.7, and 56.9 ± 3.0, respectively; all differences were significant (P < .05). Mean LCM29-positive areas for the dorsal, lateral, and ventral columns were 85.1%, 69.7%, and 50.9%, respectively. MTR and percentage LCM29-positive area were significantly correlated (r2 = 0.98). CONCLUSION: Regional differences in MT effect exist in the lamprey spinal cord. MTR is well correlated with percentage LCM29-positive area. These results support the hypothesis that membrane constituents are at least partly responsible for regional variations in MT effect.

AB - BACKGROUND AND PURPOSE: Membrane constituents may play a key role in the magnetization transfer (MT) effect. In lamprey spinal cord, axonal diameters range from <1 μm in the dorsal region to 20-40 μm in the ventral region. There is a corresponding range of axonal, and hence cell membrane, density. These characteristics permit determination of the effect of cell membrane density on MT. The purpose of this study was to characterize regional MT effects in lamprey spinal cord. METHODS: Excised spinal cords from eight sea lampreys were measured with a 9.4-T MR imaging system. MT saturation was applied for spin-echo sequences. The MT ratio (MTR) was calculated in each location (dorsal, lateral, and ventral columns). Spinal cords from five other lampreys were prepared with an antibody to lamprey glial keratin (LCM 29). The percentage of area staining with LCM29 was calculated for each location. RESULTS: Mean MTR (± SD) for the dorsal, lateral, and ventral columns were 62.4 ± 4.2, 59.2 ± 2.7, and 56.9 ± 3.0, respectively; all differences were significant (P < .05). Mean LCM29-positive areas for the dorsal, lateral, and ventral columns were 85.1%, 69.7%, and 50.9%, respectively. MTR and percentage LCM29-positive area were significantly correlated (r2 = 0.98). CONCLUSION: Regional differences in MT effect exist in the lamprey spinal cord. MTR is well correlated with percentage LCM29-positive area. These results support the hypothesis that membrane constituents are at least partly responsible for regional variations in MT effect.

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