Maize C4 and non-C4 NADP-dependent malic enzymes are encoded by distinct genes derived from a plastid-localized ancestor

S. Lorraine Tausta, Heather Miller Coyle, Beverly Rothermel, Virginia Stiefel, Timothy Nelson

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

NADP-dependent malic enzymes (NADP-ME; EC1.1.1.40) have been implicated in a wide range of metabolic pathways in the plastids and cytosol of plant cells. In maize, an NADP-ME type C4 plant, the most abundant NADP-ME form is the chloroplastic leaf isoform that delivers CO2 intracellularly to ribulose bisphosphate carboxylase (RuBPCase). A second NADP-ME isoform predominates in maize roots and exhibits distinct C3-like enzymatic characteristics. We show that the C3-like isoform is encoded by a pair of nearly identical genes that encode precursor proteins with functional chloroplast transit peptides. Using RT-PCR, we also show that the messages encoding the C4 and C3-like NADP-ME isoforms are differentially regulated with respect to the developmental stage of the leaf, light conditions, and tissue type. Based on these characteristics and on sequence comparison of ME families in other species, we propose a scheme for the origin of the C4-specific NADP-ME gene.

Original languageEnglish (US)
Pages (from-to)635-652
Number of pages18
JournalPlant Molecular Biology
Volume50
Issue number4-5
DOIs
StatePublished - Nov 2002

Fingerprint

malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)
Plastids
malic enzyme
NADP
Zea mays
plastids
ancestry
Genes
corn
C4 plants
Protein Isoforms
ribulose-bisphosphate carboxylase
Enzymes
cytosol
biochemical pathways
leaves
genes
chloroplasts
reverse transcriptase polymerase chain reaction
developmental stages

Keywords

  • C4 photosynthesis
  • Chloroplast transit peptide
  • Malic enzyme
  • Zea mays

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Maize C4 and non-C4 NADP-dependent malic enzymes are encoded by distinct genes derived from a plastid-localized ancestor. / Tausta, S. Lorraine; Miller Coyle, Heather; Rothermel, Beverly; Stiefel, Virginia; Nelson, Timothy.

In: Plant Molecular Biology, Vol. 50, No. 4-5, 11.2002, p. 635-652.

Research output: Contribution to journalArticle

Tausta, S. Lorraine ; Miller Coyle, Heather ; Rothermel, Beverly ; Stiefel, Virginia ; Nelson, Timothy. / Maize C4 and non-C4 NADP-dependent malic enzymes are encoded by distinct genes derived from a plastid-localized ancestor. In: Plant Molecular Biology. 2002 ; Vol. 50, No. 4-5. pp. 635-652.
@article{b2d29ca635fc49a9a56e9857a968a4a8,
title = "Maize C4 and non-C4 NADP-dependent malic enzymes are encoded by distinct genes derived from a plastid-localized ancestor",
abstract = "NADP-dependent malic enzymes (NADP-ME; EC1.1.1.40) have been implicated in a wide range of metabolic pathways in the plastids and cytosol of plant cells. In maize, an NADP-ME type C4 plant, the most abundant NADP-ME form is the chloroplastic leaf isoform that delivers CO2 intracellularly to ribulose bisphosphate carboxylase (RuBPCase). A second NADP-ME isoform predominates in maize roots and exhibits distinct C3-like enzymatic characteristics. We show that the C3-like isoform is encoded by a pair of nearly identical genes that encode precursor proteins with functional chloroplast transit peptides. Using RT-PCR, we also show that the messages encoding the C4 and C3-like NADP-ME isoforms are differentially regulated with respect to the developmental stage of the leaf, light conditions, and tissue type. Based on these characteristics and on sequence comparison of ME families in other species, we propose a scheme for the origin of the C4-specific NADP-ME gene.",
keywords = "C4 photosynthesis, Chloroplast transit peptide, Malic enzyme, Zea mays",
author = "Tausta, {S. Lorraine} and {Miller Coyle}, Heather and Beverly Rothermel and Virginia Stiefel and Timothy Nelson",
year = "2002",
month = "11",
doi = "10.1023/A:1019998905615",
language = "English (US)",
volume = "50",
pages = "635--652",
journal = "Plant Molecular Biology",
issn = "0167-4412",
publisher = "Springer Netherlands",
number = "4-5",

}

TY - JOUR

T1 - Maize C4 and non-C4 NADP-dependent malic enzymes are encoded by distinct genes derived from a plastid-localized ancestor

AU - Tausta, S. Lorraine

AU - Miller Coyle, Heather

AU - Rothermel, Beverly

AU - Stiefel, Virginia

AU - Nelson, Timothy

PY - 2002/11

Y1 - 2002/11

N2 - NADP-dependent malic enzymes (NADP-ME; EC1.1.1.40) have been implicated in a wide range of metabolic pathways in the plastids and cytosol of plant cells. In maize, an NADP-ME type C4 plant, the most abundant NADP-ME form is the chloroplastic leaf isoform that delivers CO2 intracellularly to ribulose bisphosphate carboxylase (RuBPCase). A second NADP-ME isoform predominates in maize roots and exhibits distinct C3-like enzymatic characteristics. We show that the C3-like isoform is encoded by a pair of nearly identical genes that encode precursor proteins with functional chloroplast transit peptides. Using RT-PCR, we also show that the messages encoding the C4 and C3-like NADP-ME isoforms are differentially regulated with respect to the developmental stage of the leaf, light conditions, and tissue type. Based on these characteristics and on sequence comparison of ME families in other species, we propose a scheme for the origin of the C4-specific NADP-ME gene.

AB - NADP-dependent malic enzymes (NADP-ME; EC1.1.1.40) have been implicated in a wide range of metabolic pathways in the plastids and cytosol of plant cells. In maize, an NADP-ME type C4 plant, the most abundant NADP-ME form is the chloroplastic leaf isoform that delivers CO2 intracellularly to ribulose bisphosphate carboxylase (RuBPCase). A second NADP-ME isoform predominates in maize roots and exhibits distinct C3-like enzymatic characteristics. We show that the C3-like isoform is encoded by a pair of nearly identical genes that encode precursor proteins with functional chloroplast transit peptides. Using RT-PCR, we also show that the messages encoding the C4 and C3-like NADP-ME isoforms are differentially regulated with respect to the developmental stage of the leaf, light conditions, and tissue type. Based on these characteristics and on sequence comparison of ME families in other species, we propose a scheme for the origin of the C4-specific NADP-ME gene.

KW - C4 photosynthesis

KW - Chloroplast transit peptide

KW - Malic enzyme

KW - Zea mays

UR - http://www.scopus.com/inward/record.url?scp=0036836926&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036836926&partnerID=8YFLogxK

U2 - 10.1023/A:1019998905615

DO - 10.1023/A:1019998905615

M3 - Article

C2 - 12374297

AN - SCOPUS:0036836926

VL - 50

SP - 635

EP - 652

JO - Plant Molecular Biology

JF - Plant Molecular Biology

SN - 0167-4412

IS - 4-5

ER -