Maize NADP-malate dehydrogenase: cDNA cloning, sequence, and mRNA characterization

Mary C. Metzler, Beverly A. Rothermel, Timothy Nelson

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

We report here the isolation, characterization and nucleotide sequence of clones encoding the maize chloroplastic NADP-malate dehydrogenase (NADP-MDH) which functions in the C4 cycle of photosynthesis. A nearly full-length NADP-MDH cDNA clone was isolated using antibodies against the purified protein. This clone hybridizes to a 1600 base mRNA that is eight times more abundant in light-grown maize leaves than in etiolated leaves. Transcription in leaves begins 230 bp upstream of the predicted start of translation, as shown by primer extension experiments. The encoded amino acid sequence predicts that NADP-MDH is synthesized as a preprotein of 432 amino acids (46 865 Da) which is processed into a mature protein of 375 amino acids (40 934 Da) with removal of a 57 amino acid transit peptide (5 931 Da). We identify regions of similarity between the maize NADP-MDH and other MDH polypeptides.

Original languageEnglish (US)
Pages (from-to)713-722
Number of pages10
JournalPlant Molecular Biology
Volume12
Issue number6
DOIs
StatePublished - Jun 1989

Fingerprint

Malate Dehydrogenase (NADP+)
malate dehydrogenase (NADP)
Cloning
Zea mays
Organism Cloning
molecular cloning
Complementary DNA
Amino Acids
Messenger RNA
corn
Clone Cells
clones
amino acids
leaves
Peptides
Photosynthesis
Transcription
translation (genetics)
Amino Acid Sequence
polypeptides

Keywords

  • C4 cycle
  • cDNA sequence
  • maize
  • NADP-malate dehydrogenase
  • Transit peptide

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Maize NADP-malate dehydrogenase : cDNA cloning, sequence, and mRNA characterization. / Metzler, Mary C.; Rothermel, Beverly A.; Nelson, Timothy.

In: Plant Molecular Biology, Vol. 12, No. 6, 06.1989, p. 713-722.

Research output: Contribution to journalArticle

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