Mammalian achaete-scute homolog 1 is transiently expressed by spatially restricted subsets of early neuroepithelial and neural crest cells

Li Ching Lo, Jane E. Johnson, Carol W. Wuenschell, Tetsuichiro Saito, David J. Anderson

Research output: Contribution to journalArticle

348 Citations (Scopus)

Abstract

Using monoclonal antibodies, we have examined the expression pattern of MASH1, a basic helix-loop-helix protein that is a mammalian homolog of the Drosophila achaete-scute proteins. In Drosophila, achaete-scute genes are required for the determination of a subset of neurons. In the rat embryo, MASH1 expression is confined to subpopulations of neural precursor cells. The induction of MASH1 precedes, but is extinguished upon, overt neuronal differentiation. MASH1 is expressed in the forebrain by spatially restricted domains of neuroepithelium and in the peripheral nervous system exclusively by precursors of sympathetic and enteric neurons. The features of early and transient expression, in spatially restricted subpopulations of neural precursors, are similar to those observed for achaete-scute. Thus, the amino acid sequence conservation between MASH1 and achaete-scute is reflected in a parallel conservation of cell type specificity of expression, similar to the case of mammalian MyoD and Drosophila nautilus. These data support the idea that helix-loop-helix proteins may represent an evolutionary conserved family of cell-type determination genes, of which MASH1 is the first neural-specific member identified in vertebrates.

Original languageEnglish (US)
Pages (from-to)1524-1537
Number of pages14
JournalGenes and Development
Volume5
Issue number9
StatePublished - Sep 1991

Fingerprint

Neural Crest
Drosophila
Nautilus
Neurons
Peripheral Nervous System
Prosencephalon
Genes
Vertebrates
Amino Acid Sequence
Proteins
Embryonic Structures
Monoclonal Antibodies

Keywords

  • achaete-scute genes
  • Forebrain
  • Helix-loop-helix proteins
  • Neural crest development
  • Neuronal determination
  • Nuclear regulatory factor

ASJC Scopus subject areas

  • Developmental Biology
  • Genetics

Cite this

Mammalian achaete-scute homolog 1 is transiently expressed by spatially restricted subsets of early neuroepithelial and neural crest cells. / Lo, Li Ching; Johnson, Jane E.; Wuenschell, Carol W.; Saito, Tetsuichiro; Anderson, David J.

In: Genes and Development, Vol. 5, No. 9, 09.1991, p. 1524-1537.

Research output: Contribution to journalArticle

Lo, Li Ching ; Johnson, Jane E. ; Wuenschell, Carol W. ; Saito, Tetsuichiro ; Anderson, David J. / Mammalian achaete-scute homolog 1 is transiently expressed by spatially restricted subsets of early neuroepithelial and neural crest cells. In: Genes and Development. 1991 ; Vol. 5, No. 9. pp. 1524-1537.
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