Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways

Yi Wei Jiang, Philippe Veschambre, Hediye Erdjument-Bromage, Paul Tempst, Joan W. Conaway, Ronald C. Conaway, Roger D. Kornberg

Research output: Contribution to journalArticlepeer-review

257 Scopus citations

Abstract

A multiprotein complex isolated from murine cells is identified as a counterpart of the yeast Mediator of transcriptional regulation on the basis of the following: homologs of two subunits of yeast Mediator, Srb7 and Med7, copurify with the complex; peptide sequencing reveals, in addition, homologs of the yeast Mediator subunits Rgr1 and Med6; as with yeast Mediator, the mouse complex binds to the RNA polymerase II C-terminal domain (CTD) and stimulates phosphorylation of the CTD by TFIIH. Peptide sequencing also identifies a component of mouse Mediator as a relative of Ring-3 protein, a mitogen-activated nuclear protein kinase, raising the possibility of Mediator as an end point of signal transduction pathways.

Original languageEnglish (US)
Pages (from-to)8538-8543
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number15
DOIs
StatePublished - Jul 21 1998
Externally publishedYes

Keywords

  • C-terminal domain
  • MED genes
  • Ring-3
  • SRB genes
  • TFIIH kinase

ASJC Scopus subject areas

  • General

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